Screening method for the detection of a range of nitrofurans in avian eyes by optical biosensor
| Autor: | Imelda M. Traynor, Terence L. Fodey, Steven R. H. Crooks, Colin S. Thompson, D. Glenn Kennedy |
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| Rok vydání: | 2011 |
| Předmět: |
Furazolidone
Nitrofurans medicine.drug_class Biosensing Techniques Cross Reactions Eye Biochemistry Antibodies Analytical Chemistry Birds Furaltadone medicine Animals Environmental Chemistry Solid phase extraction Nitrofuran Spectroscopy Immunoassay Nitrofurazone Chromatography Chemistry Solid Phase Extraction Optical biosensor Nitrofurantoin Biosensor medicine.drug |
| Zdroj: | Analytica Chimica Acta. 700:177-182 |
| ISSN: | 0003-2670 |
| Popis: | An immunobiosensor assay was developed for the multi-residue screening of a range of nitrofuran compounds in avian eyes. A polyclonal antibody which binds at least 5 of the major parent nitrofurans was raised in a rabbit after inoculation with a nitrofuran mimic-protein conjugate. Sample homogenates were extracted into 0.1 M hydrochloric acid and subjected to clean-up by solid phase extraction and micro-centrifugation prior to biosensor analysis. Validation data obtained from the analysis of 21 fortified samples has shown that the method has a detection capability (CCβ) of less than 1 ng eye−1 for nitrofurazone (NFZ). In addition, cross-reactivity data and the analysis of a smaller number of fortified samples have shown that the method will also detect a range of other major parent nitrofurans including furazolidone (FZD), furaltadone (FTD), nitrofurantoin (NFA) and nifursol (NFS). Intra-assay variation (n = 10) was calculated at 12.9% and 10.1% at concentrations of 1 ng eye−1 and 2 ng eye−1 NFZ respectively. Inter-assay variation (n = 3) was determined to be 10.8% and 4.7% at the same NFZ concentrations respectively. The cross-reactivity profile and validation data for the detection of these nitrofurans are presented together with the results obtained following the analysis of a small number of incurred samples using the developed method. |
| Databáze: | OpenAIRE |
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