In Vivo Effects of Molybdate on Activation of Rat Liver Cytosol Glucocorticoid Receptor
| Autor: | Yasuo Mitani, Chiaki Shigemasa, Keita Urabe, Y. Ueda, Akio Yoshida, Toshiaki Adachi, Kyoju Abe, Hiroto Mashiba, S. T. Taniguchi |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
medicine.medical_specialty Hydrocortisone Endocrinology Diabetes and Metabolism Sodium molybdate Clinical Biochemistry Biochemistry Inferior vena cava Dexamethasone chemistry.chemical_compound Cytosol Receptors Glucocorticoid Endocrinology Glucocorticoid receptor In vivo Internal medicine medicine Animals Molybdenum Biochemistry (medical) Rats Inbred Strains General Medicine Rats Liver medicine.vein chemistry Hormone receptor Glucocorticoid Hormone medicine.drug |
| Zdroj: | Hormone and Metabolic Research. 20:566-569 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-2007-1010886 |
| Popis: | To clarify the in vivo events following administration of steroid hormones, the effect of molybdate on the cytoplasmic glucocorticoid receptor (GR) translocation in rat livers after administration of glucocorticoids was examined. After injection of hydrocortisone-succinate (Hydro) (200 micrograms/150 g rat) into the inferior vena cava of adrenalectomized rats, 3H-dexamethasone (3H-Dex) binding in liver cytosol rapidly decreased within 5 min, followed by a rapid replenishment. This decrease in binding was partially prevented by pre-treating the animals with 20 mg of sodium molybdate (Mo), which was considered to inhibit GR activation. Administration of 200 micrograms/150 g body weight of hydrocortisone caused a rapid replenishment of the binding sites following the depletion. Administration of molybdate 5 min after the hydrocortisone injection did not influence the replenishment. The GR content was increased significantly 20 min after the administration of molybdate in intact rats but not in adrenalectomized rat. It is concluded that activation of the hormone receptor complex observed in vitro also occurs in vivo after administration of glucocorticoid to animals. |
| Databáze: | OpenAIRE |
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