Purification of megakaryocyte differentiation activity from a human fibrous histiocytoma cell line: N-terminal sequence homology with activin A
| Autor: | Tetsuya Masuda, Hideki Sasaki, Tohru Inoue, Kazuharu Kato, Kiyoshi Takatsuki, Jiro Hirose, Mitsuko Isaji, Hiromitsu Matsuzaki, Yuji Yonemura, Makoto Kawakita, Koji Fujimoto |
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| Rok vydání: | 1991 |
| Předmět: |
endocrine system
Macromolecular Substances Megakaryocyte differentiation Cellular differentiation Molecular Sequence Data Biophysics Biology Biochemistry Cell Line Mice Megakaryocyte Sequence Homology Nucleic Acid Homologous chromosome medicine Animals Humans Inhibins Amino Acid Sequence Molecular Biology Polyacrylamide gel electrophoresis Peptide sequence Histiocytoma Benign Fibrous Cell Differentiation Cell Biology Molecular biology Activins medicine.anatomical_structure Cell culture Immunology Megakaryocytes hormones hormone substitutes and hormone antagonists ACVR2B |
| Zdroj: | Biochemical and Biophysical Research Communications. 174:1163-1168 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(91)91543-l |
| Popis: | We purified a protein possessing a potent ability to induce the differentiation of a murine megakaryoblastic cell line, L8057, from the supernatant of a human fibrous histiocytoma cell line, KHM-5M. The protein, a homodimer of a molecular weight of 25-kDa, has an N-terminal sequence identical with that of the beta A-chain of inhibin, indicating that it is identical to or highly homologous with activin A. Thus, it is speculated that activin A or its homologue is involved in megakaryocytic differentiation. |
| Databáze: | OpenAIRE |
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