Expression of plasmolipin in oligodendrocytes
| Autor: | J. T. Konola, Elizabeth F. Cochary, Itzhak Fischer, Giuseppina Romano-Clarke |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Proteolipids
Fluorescent Antibody Technique Galactosylceramides Nerve Tissue Proteins Mice Cellular and Molecular Neuroscience Myelin medicine Animals Myelin proteolipid Cells Cultured Glial fibrillary acidic protein biology Myelin and Lymphocyte-Associated Proteolipid Proteins Membrane Proteins Myelin Basic Protein GFAP stain Molecular biology Oligodendrocyte Rats Staining Oligodendroglia medicine.anatomical_structure Gene Expression Regulation Cell culture Astrocytes Immunology biology.protein Neuroglia |
| Zdroj: | Journal of Neuroscience Research. 28:81-89 |
| ISSN: | 1097-4547 0360-4012 |
| Popis: | Plasmolipin is a plasma membrane proteolipid which has recently been described as a component of myelin (Cochary et al.: Journal of Neurochemistry 55:602-610, 1990). The present study reports the expression and localization of plasmolipin in primary glial cultures and secondary oligodendrocyte cultures. Double-label immunofluorescence showed that plasmolipin was expressed by galactocerebroside (GC)-positive oligodendrocytes, but was absent from astrocytes, characterized by their positive staining for glial fibrillary acidic protein (GFAP). At 1 week in culture plasmolipin staining was relatively weak in the cell body of some of the GC-positive cells. During the following 3 weeks in culture plasmolipin staining of oligodendrocytes gradually increased and was present in the cell body, its plasma membrane, and all the processes. However, the plasmolipin antibodies did not stain regions of the flat membrane sheets. Western blot analysis of homogenates from primary glial cultures showed that plasmolipin levels gradually increased during the first 5 weeks in culture. We conclude that the presence of plasmolipin in myelin is a result of its expression by oligodendrocytes. |
| Databáze: | OpenAIRE |
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