Prognostic Significance of Circulating Tumor Cells with Mesenchymal Phenotypes in Patients with Gastric Cancer: A Prospective Study
| Autor: | Tadashi Yoshida, Norihiko Takahashi, Akinobu Taketomi, Shigenori Homma, Hideki Kawamura, Moto Fukai, Yui Ishiguro, Nobuki Ichikawa, Hideyasu Sakihama |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Epithelial-Mesenchymal Transition Gastroenterology 03 medical and health sciences Cytokeratin 0302 clinical medicine Circulating tumor cell Stomach Neoplasms Surgical oncology Internal medicine Biomarkers Tumor medicine Humans Prospective Studies Prospective cohort study Survival rate business.industry Mesenchymal stem cell Cancer Perioperative Neoplastic Cells Circulating Prognosis medicine.disease Phenotype Oncology 030220 oncology & carcinogenesis 030211 gastroenterology & hepatology Surgery Neoplasm Recurrence Local business |
| Zdroj: | Annals of Surgical Oncology. 28:1178-1186 |
| ISSN: | 1534-4681 1068-9265 |
| Popis: | Circulating tumor cells (CTCs) have been shown to be heterogeneous. Focusing on the epithelial–mesenchymal transition and perioperative kinetics, we evaluated CTCs with mesenchymal phenotypes as a potential prognostic biomarker for patients with gastric cancer. Peripheral blood was collected from 54 patients with gastric cancer before surgery and at 1 week and 1 month after surgery. CTCs were enriched using density-gradient centrifugation and magnetic-activated cell sorting (negative selection). Cell suspensions were characterized by multi-immunofluorescence staining against cytokeratin and N-cadherin, and by 4′,6′-diamidino-2-phenyldole staining. CTCs were detected in five patients (17%) with early cancer and 14 patients (56%) with advanced cancer (p |
| Databáze: | OpenAIRE |
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