Host origin determines pH tolerance of Tritrichomonas foetus isolates from the feline gastrointestinal and bovine urogenital tracts
| Autor: | Colin M. Stack, Victoria Morin-Adeline, Stuart T. Fraser, Jan Šlapeta |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Genotype Immunology Cell Cattle Diseases Urogenital System Tritrichomonas foetus Biology Cat Diseases Cell morphology Host-Parasite Interactions Microbiology Flow cytometry Microscopy Electron Transmission medicine Extracellular Animals Parasite hosting Protozoan Infections Animal Fluorescent Dyes medicine.diagnostic_test General Medicine Hydrogen-Ion Concentration Flow Cytometry biology.organism_classification Adaptation Physiological Gastrointestinal Tract Infectious Diseases medicine.anatomical_structure Cytoplasm Cats Cattle Parasitology |
| Zdroj: | Experimental Parasitology. 157:68-77 |
| ISSN: | 0014-4894 |
| Popis: | The ability for protozoan parasites to tolerate pH fluctuations within their niche is critical for the establishment of infection and require the parasite to be capable of adapting to a distinct pH range. We used two host adapted Tritrichomonas foetus isolates, capable of infecting either the digestive tract (pH 5.3-6.6) of feline hosts or the reproductive tract (pH 7.4-7.8) of bovine hosts to address their adaptability to changing pH. Using flow cytometry, we investigated the pH tolerance of the bovine and feline T. foetus isolates over a range of physiologically relevant pH in vitro. Following exposure to mild acid stress (pH 6), the bovine T. foetus isolates showed a significant decrease in cell viability and increased cytoplasmic granularity (p-value 0.003, p-value 0.0002) compared to pH 7 and 8 (p-value 0.7). In contrast, the feline genotype displayed an enhanced capacity to maintain cell morphology and viability (p-value 0.05). Microscopic assessment revealed that following exposure to a weak acidic stress (pH 6), the bovine T. foetus transformed into rounded parasites with extended cell volumes and displays a decrease in viability. The higher tolerance for acidic extracellular environment of the feline isolate compared to the bovine isolate suggests that pH could be a critical factor in regulating T. foetus infections and host-specificity. |
| Databáze: | OpenAIRE |
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