Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults
| Autor: | S. Raes, Jozef Vercruysse, Mark Blaxter, Dirk C. de Graaf, Luc Moens, Paul Berghen, T. De Marez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Trichostrongylus
nematode Blotting Western Molecular Sequence Data Antibodies Helminth Enzyme-Linked Immunosorbent Assay Biology Antigen Western blot medicine Animals Parasite hosting Amino Acid Sequence Globin Fluorescent Antibody Technique Indirect Ostertagia ostertagi Molecular mass medicine.diagnostic_test Immune Sera Immunogenicity Ostertagia Chromatography Ion Exchange biology.organism_classification Molecular biology haemoglobin Globins Molecular Weight Infectious Diseases Nematode Antigens Helminth Chromatography Gel Cattle Animal Science and Zoology Parasitology Nippostrongylus Rabbits Sequence Alignment |
| Zdroj: | de Graaf, D C, Berghen, P, Moens, L, de Marez, T M, Raes, S, Blaxter, M L & Vercruysse, J 1996, ' Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults ', Parasitology, vol. 113, no. 1, pp. 63-69 . https://doi.org/10.1017/S0031182000066282 Parasitology |
| ISSN: | 0031-1820 |
| DOI: | 10.1017/S0031182000066282 |
| Popis: | SUMMARYWestern blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts ofOstertagia ostertagiadults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle ofO. ostertagiadults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected byO. ostertagiand/orCooperia oncophoraand the negative controls. |
| Databáze: | OpenAIRE |
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