Molecular characterization of the first saltwater crocodilepox virus genome sequences from the world's largest living member of the Crocodylia
| Autor: | Peter Lock, Karla J. Helbig, Subir Sarker, Natalie L. Milic, Sally R. Isberg |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient 040301 veterinary sciences lcsh:Medicine Genomics Genome Viral Poxviridae Infections Crocodile Genome Skin Diseases Article 0403 veterinary science 03 medical and health sciences Monophyly food Phylogenetics biology.animal Animals lcsh:Science Gene Phylogeny Uncategorized Genetics Alligators and Crocodiles Multidisciplinary Phylogenetic tree biology Virulence lcsh:R Crocodylidpoxvirus Australia 04 agricultural and veterinary sciences Sequence Analysis DNA 030104 developmental biology lcsh:Q Chordopoxvirinae |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Crocodilepox virus is a large dsDNA virus belonging to the genus Crocodylidpoxvirus, which infects a wide range of host species in the order Crocodylia worldwide. Here, we present genome sequences for a novel saltwater crocodilepox virus, with two subtypes (SwCRV-1 and -2), isolated from the Australian saltwater crocodile. Affected belly skins of juvenile saltwater crocodiles were used to sequence complete viral genomes, and perform electron microscopic analysis that visualized immature and mature virions. Analysis of the SwCRV genomes showed a high degree of sequence similarity to CRV (84.53% and 83.70%, respectively), with the novel SwCRV-1 and -2 complete genome sequences missing 5 and 6 genes respectively when compared to CRV, but containing 45 and 44 predicted unique genes. Similar to CRV, SwCRV also lacks the genes involved in virulence and host range, however, considering the presence of numerous hypothetical and or unique genes in the SwCRV genomes, it is completely reasonable that the genes encoding these functions are present but not recognized. Phylogenetic analysis suggested a monophyletic relationship between SwCRV and CRV, however, SwCRV is quite distinct from other chordopoxvirus genomes. These are the first SwCRV complete genome sequences isolated from saltwater crocodile skin lesions. |
| Databáze: | OpenAIRE |
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