Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Autor: Youlun Gui, Guoting Li, Jianping Zhu, Shuwu Xie, Yan Zhu, Zhao Li, Ruihua Zhong, Aying Ma
Rok vydání: 2016
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2016 (2016)
Evidence-based Complementary and Alternative Medicine : eCAM
ISSN: 1741-4288
1741-427X
Popis: We evaluated the effectiveness of Kuntai Capsule (KTC) for treating endometriosis using rat model and investigated its preliminary mechanism of action involved. SD rats were implanted with endometrial tissues and treated with KTC for three weeks. Then, laparotomy was performed to examine volume changes of the autografts. The serum levels of TNF-α, IL-6, COX-2, E2, and P4 were measured through ELISA. TUNEL was performed to analyze the apoptosis on ectopic endometrium. Protein levels of caspases 8, 9, and 3 and cytochrome c in the ectopic and eutopic endometrium were measured by western blotting. Results showed that KTC significantly decreased the volumes of ectopic endometrium. The level of TNF-αincreased and E2decreased in the KTC treatment groups. TUNEL and western blot assay showed that KTC could induce apoptosis of endometriotic tissues, accompanied with the increased protein expression of caspases 8 and 9, activated caspase-3, and cytochrome c in a dose-dependent manner. However, these protein expression profiles were not affected in eutopic endometrium. Our findings suggest that KTC could inhibit the growth of ectopic endometrial tissue through upregulating the level of TNF-αand its downstream signaling, including caspases and cytochrome c.
Databáze: OpenAIRE
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