Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model
Autor: | Youlun Gui, Guoting Li, Jianping Zhu, Shuwu Xie, Yan Zhu, Zhao Li, Ruihua Zhong, Aying Ma |
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Rok vydání: | 2016 |
Předmět: |
030219 obstetrics & reproductive medicine
TUNEL assay Article Subject biology Cytochrome Cytochrome c Endometriosis lcsh:Other systems of medicine lcsh:RZ201-999 medicine.disease Andrology Blot 03 medical and health sciences 0302 clinical medicine Complementary and alternative medicine Mechanism of action Apoptosis 030220 oncology & carcinogenesis Immunology biology.protein medicine medicine.symptom Caspase Research Article |
Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2016 (2016) Evidence-based Complementary and Alternative Medicine : eCAM |
ISSN: | 1741-4288 1741-427X |
Popis: | We evaluated the effectiveness of Kuntai Capsule (KTC) for treating endometriosis using rat model and investigated its preliminary mechanism of action involved. SD rats were implanted with endometrial tissues and treated with KTC for three weeks. Then, laparotomy was performed to examine volume changes of the autografts. The serum levels of TNF-α, IL-6, COX-2, E2, and P4 were measured through ELISA. TUNEL was performed to analyze the apoptosis on ectopic endometrium. Protein levels of caspases 8, 9, and 3 and cytochrome c in the ectopic and eutopic endometrium were measured by western blotting. Results showed that KTC significantly decreased the volumes of ectopic endometrium. The level of TNF-αincreased and E2decreased in the KTC treatment groups. TUNEL and western blot assay showed that KTC could induce apoptosis of endometriotic tissues, accompanied with the increased protein expression of caspases 8 and 9, activated caspase-3, and cytochrome c in a dose-dependent manner. However, these protein expression profiles were not affected in eutopic endometrium. Our findings suggest that KTC could inhibit the growth of ectopic endometrial tissue through upregulating the level of TNF-αand its downstream signaling, including caspases and cytochrome c. |
Databáze: | OpenAIRE |
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