Daily requirement for and splanchnic uptake of leucine in healthy adult Indians
| Autor: | Kalburgi Maruthy, Vernon R Young, Rebecca Kuriyan, Deepak Chandukudlu, Anura V Kurpad, Antoine E. El-Khoury, Tony Raj, Sudhir Borgonha, Meredith M. Regan |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Nitrogen balance medicine.medical_specialty Nitrogen India Medicine (miscellaneous) Random order Animal science Leucine Internal medicine medicine Humans Infusions Intravenous Carbon Isotopes Nutrition and Dietetics Chemistry Nutritional Requirements Calorimetry Indirect Oxidation reduction Carbon Dioxide Viscera Endocrinology Breath Tests Leucine metabolism Regression Analysis Leucine intake Splanchnic Oxidation-Reduction |
| Zdroj: | The American Journal of Clinical Nutrition. 74:747-755 |
| ISSN: | 0002-9165 |
| DOI: | 10.1093/ajcn/74.6.747 |
| Popis: | Background The 1985 FAO/WHO/UNU requirement for leucine is too low according to tracer-derived estimates of leucine oxidation and balance in adults from developed regions. Objective The leucine requirement in populations in developing countries was assessed with use of the 24-h tracer balance method and on the basis of nitrogen balances. Design Twenty healthy Indian men were studied during their consumption for 6 d of 2 L-amino acid diets that supplied either 14 and 30 (n = 10) or 22 and 40 (n = 10) mg leucine x kg(-1) x d(-1) in random order. At 1800 on day 7, a 24-h constant intravenous [13C]leucine tracer-infusion protocol was conducted to determine leucine oxidation and daily leucine balance. During the intake of 40 mg leucine/d, [2H3]leucine was given orally to assess the splanchnic uptake of leucine. Results Mean 24-h leucine oxidation rates were 29.8, 30.6, 33.6, and 39.3 mg x kg(-1) x d(-1) at leucine intakes of 14, 22, 30, and 40 mg x kg(-1) x d(-1), respectively; daily leucine balances were -16.5, -9.0, -3.3, and 0.5 mg x kg(-1) x d(-1), respectively. Mixed-models linear regression of balance against leucine intake resulted in a zero balance at a leucine intake of 37.3 mg x kg(-1) x d(-1). Nitrogen balances were -12.7, -17.9, -3.9, and 1.0 mg x kg(-1) x d(-1) at leucine intakes of 14, 22, 30, and 40 mg x kg(-1) x d(-1). Regression of nitrogen balance against intake resulted in a zero balance at a leucine intake of 37.6 mg x kg(-1) x d(-1). The first-pass splanchnic uptake of leucine was 45.7% and 33.9% in the fasted and fed periods, respectively. Conclusion A tentative mean leucine requirement of 40 mg x kg(-1) x d(-1) is proposed for healthy Indian adults, as it is for Western subjects. |
| Databáze: | OpenAIRE |
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