Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

Autor:	Ioanna Chinou, Anna Kawiak, M Jeziorek, Anna Gawron, Ewa Łojkowska, Katarzyna Sykłowska-Baranek, Agnieszka Pietrosiuk
Rok vydání:	2011
Předmět:	Methyl jasmonate Isobutyrylshikonin biology Transgene Plant physiology Horticulture biology.organism_classification medicine.disease Molecular biology Lithospermum canescens chemistry.chemical_compound Leukemia chemistry Botany medicine Cytotoxic T cell Cytotoxicity
Zdroj:	Plant Cell, Tissue and Organ Culture (PCTOC). 108:213-219
ISSN:	1573-5044 0167-6857
DOI:	10.1007/s11240-011-0032-6
Popis:	The effects of medium exchange and methyl jasmonate addition on growth and production of shikonin and its derivatives acetylshikonin and isobutyrylshikonin in hairy root cultures of Lithospermum canescens were investigated. Responses varied depending on the transgenic line and stage of growth at which these lines were subjected to treatment. Shikonin itself was not detected, irrespective of the transgenic line and culture treatment used. A eightfold increase in acetylshikonin and isobutyrylshikonin accumulation was achieved when 32-day-old transgenic roots of Lc1D line were transferred from LS to M9 medium for the subsequent 3 weeks of culture. Methyl jasmonate exerted a detrimental effect on red naphthoquinones production. The extracts obtained from roots cultivated in M9 medium for 3 weeks were subjected to a cytotoxicity assay and displayed cytotoxic activity against human promyelocytic leukemia cells (HL-60) at the dose of 4 μg ml−1.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c22143df96f79516c1a381be555f0cba https://doi.org/10.1007/s11240-011-0032-6 Zobrazit plný text záznamu Full text from SpringerLink