Local and systemic inflammatory lipid profiling in a rat model of osteoarthritis with metabolic dysregulation
| Autor: | H.M. de Visser, Srinivasarao Ravipati, F.P.J.G. Lafeber, David A. Barrett, Harrie Weinans, Simon C. Mastbergen, Victoria Chapman, Ferran Casbas Pinto, P.M.J. Welsing |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Knee Joint Physiology lcsh:Medicine Knee Joints Osteoarthritis Pathology and Laboratory Medicine Biochemistry 0302 clinical medicine Tandem Mass Spectrometry Synovial Fluid Medicine and Health Sciences lcsh:Science Immune Response Musculoskeletal System Multidisciplinary Agricultural and Biological Sciences(all) Chemistry Lipids Body Fluids medicine.anatomical_structure Connective Tissue Metabolome Inflammation Mediators Anatomy medicine.symptom Research Article medicine.medical_specialty Histology Immunology Inflammation 03 medical and health sciences Signs and Symptoms Rheumatology Diagnostic Medicine Internal medicine medicine Animals Synovial fluid Oxylipins Rats Wistar Nutrition 030203 arthritis & rheumatology Biochemistry Genetics and Molecular Biology(all) Arthritis Cartilage lcsh:R Biology and Life Sciences Lipid metabolism Oxylipin Lipid Metabolism medicine.disease Rats Diet Disease Models Animal Joints (Anatomy) Biological Tissue 030104 developmental biology Endocrinology lcsh:Q Biomarkers Homeostasis Chromatography Liquid Genetics and Molecular Biology(all) |
| Zdroj: | PLoS ONE [E], 13(4). Public Library of Science PLoS ONE, 13(4) PLoS ONE, Vol 13, Iss 4, p e0196308 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Objective Bioactive oxidised lipids (oxylipins) are important signalling mediators, capable of modulating the inflammatory state of the joint and anticipated to be of importance in joint homeostasis and status of osteoarthritis. The aim of this study was to quantify oxylipin levels in plasma and synovial fluid from rats with experimentally induced osteoarthritis to investigate the potential role of oxylipins as a marker in the disease process of early osteoarthritis. Design Forty rats were randomly allocated to a standard or high-fat diet group. After 12 weeks, local cartilage damage was induced in one knee joint in 14 rats of each diet group. The remaining 6 rats per group served as controls. At week 24, samples were collected. Oxylipin levels were quantified by liquid chromatography–mass spectrometry. Results Overall, 31 lipid-derived inflammatory mediators were detected in fasted plasma and synovial fluid. Principal component analysis identified four distinct clusters associated with histopathological changes. Diet induced differences were evident for 13 individual plasma oxylipins, as well as 5,6-EET in synovial fluid. Surgical-model induced differences were evident for three oxylipins in synovial fluid (15-HETE, 8,9-DHET and 17R-ResolvinD1) with a different response in lipid concentrations for synovial fluid and plasma. Conclusions We demonstrate the quantification of oxidised lipids in rat plasma and synovial fluid in a model of early experimental osteoarthritis. Oxylipins in the synovial fluid that were altered as consequence of the surgically induced osteoarthritis were not represented in the plasma. Our findings suggest differential roles of the oxylipins in the local versus peripheral compartment. |
| Databáze: | OpenAIRE |
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