Very low concentration of cerium dioxide nanoparticles induce DNA damage, but no loss of vitality, in human spermatozoa
| Autor: | Jeanne Perrin, I. Sari-Minodier, Lise Preaubert, Mélanie Auffan, Jérôme Rose, Virginie Tassistro, Blandine Courbiere |
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| Přispěvatelé: | Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Service de Gynécologie et Obstétrique [Marseille], Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Collège de France (CdF)-Institut national des sciences de l'Univers (INSU - CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male DNA damage Cell Survival media_common.quotation_subject chemistry.chemical_element Metal Nanoparticles Human spermatozoa 010501 environmental sciences Environment Toxicology medicine.disease_cause 01 natural sciences Cerium dioxide nanoparticles 03 medical and health sciences chemistry.chemical_compound Microscopy Electron Transmission medicine Humans Internalization Comet assay 0105 earth and related environmental sciences media_common [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health General Medicine Cerium Spermatozoa In vitro 030104 developmental biology chemistry [SDV.TOX]Life Sciences [q-bio]/Toxicology Biophysics Reproductive toxicity DNA Genotoxicity |
| Zdroj: | Toxicology in Vitro Toxicology in Vitro, 2018, 50, pp.236-241. ⟨10.1016/j.tiv.2018.03.013⟩ Toxicology in Vitro, Elsevier, 2018, 50, pp.236-241. ⟨10.1016/j.tiv.2018.03.013⟩ |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2018.03.013⟩ |
| Popis: | International audience; Cerium dioxide nanoparticles (CeO 2 NP) are widely used for industrial purposes, as in diesel, paint, wood stain and as potential therapeutic applications. The Organization for Economic Cooperation and Development included CeO 2 NP in the priority list of nanomaterials requiring urgent evaluation. As metal nanoparticles can cross the blood-testis barrier, CeO 2 NP could interact with spermatozoa. The genotoxicity of CeO 2 NP was demonstrated in vitro on human cell lines and mouse gametes. However, the effects of CeO 2 NP on human spermatozoa DNA remain unknown. We showed significant DNA damage induced in vitro by CeO 2 NP on human spermatozoa using Comet assay. The genotoxicity was inversely proportional to the concentration (0.01 to 10 mg·L −1). TEM showed no internalization of CeO 2 NP into the spermatozoa. This study shows for the first time that in vitro exposure to very low concentrations of cerium dioxide nanoparticles can induce significant DNA damage in human spermatozoa. These results add new and important insights regarding the reproductive toxicity of priority nanomaterials, which require urgent evaluation. |
| Databáze: | OpenAIRE |
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