Confocal Raman microspectroscopic study of folate receptor-targeted delivery of 6-mercaptopurine-embedded gold nanoparticles in a single cell
| Autor: | Nam Woong Song, Kwang-Su Ock, Sang-Woo Joo, Erdene-Ochir Ganbold, Jin Park, Keunchang Cho, So Yeong Lee, Won Il Jeon, Ji Hye Seo, Jin-Ho Park |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Materials science Cell Survival Confocal Biomedical Engineering Metal Nanoparticles Mice Nude Spectrum Analysis Raman Absorption Biomaterials HeLa Mice Drug Delivery Systems Folic Acid Live cell imaging Animals Humans Viability assay Mice Inbred BALB C biology Mercaptopurine Folate Receptors GPI-Anchored Metals and Alloys biology.organism_classification Targeted drug delivery Biochemistry Folate receptor Colloidal gold Drug delivery Ceramics and Composites Gold HeLa Cells |
| Zdroj: | Journal of biomedical materials research. Part A. 100(5) |
| ISSN: | 1552-4965 |
| Popis: | We investigate the cellular uptake behaviors and efficacy of folate-coated gold nanoparticles (AuNPs) for the targeted drug delivery system in human cancer cells. Folate-conjugated AuNPs embedded with a purine analogue cancer drug of 6-mercaptopurine (6MP) were assembled via a 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) coupling reaction between the amino group of 4-aminobenzenethiol (ABT) and the carboxyl group of folic acid. The assembly of folate and 6MP on AuNPs has been examined by absorption spectroscopy, transmission electron microscopy (TEM), and confocal Raman spectroscopy. The internalization of the conjugated AuNPs inside the folate receptor-positive HeLa and KB cells was checked by TEM and dark-field microscopy (DFM) combined with label-free confocal spectroscopy over the depth variable z at a micrometer resolution. DFM live cell imaging of folate-conjugated AuNPs in HeLa cells indicated that the targeted AuNPs appeared to attach on the cell surfaces and enter into the cell with an hour. The cell viability was also compared to estimate the efficacy of folate-conjugated AuNP delivery systems. Folate receptor-targeted AuNP systems appeared to decrease cancer cell viability both in vitro and in vivo more than did the use of the 6MP-coated AuNPs drug without any targeting systems. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012. |
| Databáze: | OpenAIRE |
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