Methylation of macromolecules during development in Myxococcus xanthus
| Autor: | S M Panasenko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1985 |
| Předmět: |
Gel electrophoresis
Glycerol biology Cell growth Carbohydrates Methylation Carbohydrate Carbohydrate metabolism biology.organism_classification Microbiology Culture Media Kinetics Biochemistry Bacterial Proteins Protein methylation Carbohydrate Metabolism Myxococcaceae Electrophoresis Polyacrylamide Gel Myxococcales Myxococcus xanthus Molecular Biology Research Article |
| Popis: | Covalent modification of macromolecules can serve to alter their biological activities and is therefore frequently involved in regulation. I examined methylation of proteins and carbohydrates during development and vegetative growth in the procaryote Myxococcus xanthus. Striking differences in the patterns of protein methylation occurred when cell development was induced by nutrient deprivation on solid media and when cells were starved in liquid. In addition, a methylated, protease-resistant macromolecule which contained carbohydrate and which may have been an unusual type of lipopolysaccharide was observed on sodium dodecyl sulfate-polyacrylamide gels. A comparison of methylation patterns in various media and an analysis of the time course of methylation indicated that changes in methylation were part of the developmental pathway which includes aggregation. Induction of development in liquid by glycerol produced no changes in methylation. |
| Databáze: | OpenAIRE |
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