Comparison of the FilmArray RP, Verigene RV+, and Prodesse ProFLU+/FAST+ multiplex platforms for detection of influenza viruses in clinical samples from the 2011-2012 influenza season in Belgium
| Autor: | Hanne Meeuws, Lieven J. Stuyver, Marc Van Ranst, Kristiane Schmidt, Gabriela Ispas, Lieselot Houspie, Liesbeth Van Wesenbeeck, Andrea Van Immerseel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Adult Male Adolescent viruses Influenza season medicine.disease_cause Sensitivity and Specificity Virus Young Adult Human metapneumovirus Belgium Virology Transport medium Influenza Human medicine Humans Multiplex Prospective Studies Child Aged Aged 80 and over biology Respiratory tract infections Infant Newborn Infant Middle Aged biology.organism_classification Orthomyxoviridae Nasal Mucosa Molecular Diagnostic Techniques Child Preschool Enterovirus Female Rhinovirus |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X |
| Popis: | Respiratory tract infections (RTIs) are caused by a plethora of viral and bacterial pathogens. In particular, lower RTIs are a leading cause of hospitalization and mortality. Timely detection of the infecting respiratory pathogens is crucial to optimize treatment and care. In this study, three U.S. Food and Drug Administration-approved molecular multiplex platforms (Prodesse ProFLU+/FAST+, FilmArray RP, and Verigene RV+) were evaluated for influenza virus detection in 171 clinical samples collected during the Belgian 2011-2012 influenza season. Sampling was done using mid-turbinate flocked swabs, and the collected samples were stored in universal transport medium. The amount of viral RNA present in the swab samples ranged between 3.07 and 8.82 log 10 copies/ml. Sixty samples were concordant influenza A virus positive, and 8 samples were found to be concordant influenza B virus positive. Other respiratory viruses that were detected included human rhinovirus/enterovirus, respiratory syncytial virus, parainfluenza virus type 1, human metapneumovirus, and coronavirus NL63. Twenty-five samples yielded discordant results across the various assays which required further characterization by sequencing. The FilmArray RP and Prodesse ProFLU+/FAST+ assays were convenient to perform with regard to sensitivity, ease of use, and low percentages of invalid results. Although the limit of sensitivity is of utmost importance, many other factors should be taken into account in selecting the most convenient molecular diagnostic assay for the detection of respiratory pathogens in clinical samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|