Folate-linked lipoplexes for short hairpin RNA targeting claudin-3 delivery in ovarian cancer xenografts
| Autor: | Huashan Shi, Xiawei Wei, Gu He, Li Yang, Shun-Tao Luo, Xiao Liang, Yan Chen, Yiyi Yu, Fengming Gong, Xia Zhao, Wei Wang, Min Luo, Yang Yang, Guobo Shen, Tinghong Ye, Zhiyao He, Yuquan Wei, Fuchun Guo, Cui-Cui Ma, Xiangrong Song |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Oncology
medicine.medical_specialty Genetic enhancement Mice Nude Pharmaceutical Science Biology Transfection Small hairpin RNA Folic Acid Downregulation and upregulation In vivo Cell Line Tumor Internal medicine medicine Animals Claudin-3 Humans Breast RNA Small Interfering Ovarian Neoplasms Mice Inbred BALB C CLDN3 Genetic Therapy medicine.disease Gene Expression Regulation Neoplastic Tumor progression Folate receptor Cancer research Female RNA Interference Ovarian cancer |
| Zdroj: | Journal of Controlled Release. 172:679-689 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2013.10.015 |
| Popis: | Ovarian cancers highly overexpress folate receptor α (FRα) and claudin3 (CLDN3), both of which are associated with tumor progression and poor prognosis of patients. Downregulation of FRα and CLDN3 in ovarian cancer may suppress tumor growth and promote benign differentiation of tumor. In this study, F-P-LP/CLDN3, a FRα targeted liposome loading with short hairpin RNA (shRNA) targeting CLDN3 was prepared and the pharmaceutical properties were characterized. Then, the antitumor effect of F-P-LP/CLDN3 was studied in an in vivo model of advanced ovarian cancer. Compared with Control, F-P-LP/CLDN3 promoted benign differentiation of tumor and achieved about 90% tumor growth inhibition. In the meantime, malignant ascites production was completely inhibited, and tumor nodule number and tumor weight were significantly reduced (p |
| Databáze: | OpenAIRE |
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