Identification of the causative gene of a transparent phenotype of juvenile red sea bream Pagrus major
Autor: | Daiki Noguchi, Hayato Usui, Koichiro Nakano, Goro Yoshizaki, Yosuke Akiba, Yoshihiro Handa, Shuwa Sanada, Kenta Kawamoto, Eitaro Sawayama, Miwa Suzuki, Kiyoshi Asahina, Motohiro Takagi |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Thyroid Hormones Candidate gene Genetic Linkage Single-nucleotide polymorphism medicine.disease_cause 010603 evolutionary biology 01 natural sciences Article 03 medical and health sciences Genetic linkage Genetics medicine Animals Humans Missense mutation Zebrafish Gene Genetics (clinical) Mutation biology biology.organism_classification Phenotype Sea Bream 030104 developmental biology |
Zdroj: | Heredity (Edinb) |
ISSN: | 1365-2540 0018-067X |
Popis: | Deformities in cultured fish species may be genetic, and identifying causative genes is essential to expand production and maintain farmed animal welfare. We previously reported a genetic deformity in juvenile red sea bream, designated a transparent phenotype. To identify its causative gene, we conducted genome-wide linkage analysis and identified two single nucleotide polymorphisms (SNP) located on LG23 directly linked to the transparent phenotype. The scaffold on which the two SNPs were located contained two candidate genes, duox and duoxa, which are related to thyroid hormone synthesis. Four missense mutations were found in duox and one in duoxa, with that in duoxa showing perfect association with the transparent phenotype. The mutation of duoxa was suggested to affect the transmembrane structure and thyroid-related traits, including an enlarged thyroid gland and immature erythrocytes, and lower thyroxine (T(4)) concentrations were observed in the transparent phenotype. The transparent phenotype was rescued by T(4) immersion. Loss-of-function of duoxa by CRISPR–Cas9 induced the transparent phenotype in zebrafish. Evidence suggests that the transparent phenotype of juvenile red sea bream is caused by the missense mutation of duoxa and that this mutation disrupts thyroid hormone synthesis. The newly identified missense mutation will contribute to effective selective breeding of red sea bream to purge the causative gene of the undesirable phenotype and improve seed production of red sea bream as well as provide basic information of the mechanisms of thyroid hormones and its related diseases in fish and humans. |
Databáze: | OpenAIRE |
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