The protein kinase MLTK regulates chondrogenesis by inducing the transcription factor Sox6
| Autor: | Eisuke Nishida, Kei Nakayama, Toshiyasu Suzuki, Morioh Kusakabe |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
MAP Kinase Signaling System
Xenopus Regulator SOX9 Xenopus Proteins Xenopus laevis Chondrocytes Cell Movement Animals Protein kinase A Molecular Biology Transcription factor In Situ Hybridization DNA Primers Oligonucleotide Array Sequence Analysis Gene knockdown biology Base Sequence Gene Expression Regulation Developmental Cell Differentiation Anatomy biology.organism_classification Chondrogenesis MAP Kinase Kinase Kinases Cell biology Neural Crest Gene Knockdown Techniques Ectopic expression SOXD Transcription Factors Developmental Biology |
| Zdroj: | Development (Cambridge, England). 139(16) |
| ISSN: | 1477-9129 |
| Popis: | Sox9 acts together with Sox5 or Sox6 as a master regulator for chondrogenesis; however, the inter-relationship among these transcription factors remains unclear. Here, we show that the protein kinase MLTK plays an essential role in the onset of chondrogenesis through triggering the induction of Sox6 expression by Sox9. We find that knockdown of MLTK in Xenopus embryos results in drastic loss of craniofacial cartilages without defects in neural crest development. We also find that Sox6 is specifically induced during the onset of chondrogenesis, and that the Sox6 induction is inhibited by MLTK knockdown. Remarkably, Sox6 knockdown phenocopies MLTK knockdown. Moreover, we find that ectopic expression of MLTK induces Sox6 expression in a Sox9-dependent manner. Our data suggest that p38 and JNK pathways function downstream of MLTK during chondrogenesis. These results identify MLTK as a novel key regulator of chondrogenesis, and reveal its action mechanism in chondrocyte differentiation during embryonic development. |
| Databáze: | OpenAIRE |
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