Analytical and biological methods for probing the blood-brain barrier
| Autor: | Susan M. Lunte, Kenneth L. Audus, Pradyot Nandi, Courtney D. Kuhnline Sloan, Jane V. Aldrich, Thomas H. Linz |
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| Rok vydání: | 2012 |
| Předmět: |
Microdialysis
Central nervous system Cell Culture Techniques Endogeny Pharmacology Blood–brain barrier Mass Spectrometry Article Analytical Chemistry In vivo Extracellular fluid medicine Animals Humans Chemistry Brain Electrophoresis Capillary Biological Transport Equipment Design Microfluidic Analytical Techniques medicine.disease Perfusion medicine.anatomical_structure Blood-Brain Barrier Positron-Emission Tomography Injections Intravenous Infiltration (medical) Chromatography Liquid |
| Zdroj: | Annual review of analytical chemistry (Palo Alto, Calif.). 5 |
| ISSN: | 1936-1335 |
| Popis: | The blood-brain barrier (BBB) is an important interface between the peripheral and central nervous systems. It protects the brain against the infiltration of harmful substances and regulates the permeation of beneficial endogenous substances from the blood into the extracellular fluid of the brain. It can also present a major obstacle in the development of drugs that are targeted for the central nervous system. Several methods have been developed to investigate the transport and metabolism of drugs, peptides, and endogenous compounds at the BBB. In vivo methods include intravenous injection, brain perfusion, positron emission tomography, and microdialysis sampling. Researchers have also developed in vitro cell-culture models that can be employed to investigate transport and metabolism at the BBB without the complication of systemic involvement. All these methods require sensitive and selective analytical methods to monitor the transport and metabolism of the compounds of interest at the BBB. |
| Databáze: | OpenAIRE |
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