Human immunodeficiency virus-1 infection protects against a Tc1-to-Tc2 shift in CD8(+) T cells
| Autor: | Sowmya Balasubramanian, Janina Q. Jiang, Bilge Diker, Naveed Gulzar, Karen F.T. Copeland |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD3 Complex
T cell Immunology HIV Infections Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Virus Replication CCL5 Interleukin 21 T-Lymphocyte Subsets medicine Immunology and Allergy Cytotoxic T cell Humans IL-2 receptor Phytohemagglutinins Antigen-presenting cell Th1-Th2 Balance Cells Cultured Interleukin 3 Inflammation Antibodies Monoclonal General Medicine Molecular biology medicine.anatomical_structure Interleukin 12 Disease Progression HIV-1 Cytokines |
| Zdroj: | Human immunology. 72(11) |
| ISSN: | 1879-1166 |
| Popis: | Despite the reports of dysfunction of the lytic abilities of CD8 + T cells during human immunodeficiency virus-1 (HIV-1) disease progression, the effects of infection on the noncytolytic functions of CD8 + T cells have not been well characterized to date. We examined the effect of HIV-1 infection on the cytokine and chemokine responses of peripheral blood–derived CD8 + T cells in an in vitro system. Activation of HIV-1-infected CD8 + T cells with phytohemagglutinin resulted in a 4- to 8-fold increase in the production of macrophage inflammatory protein (MIP)-1α, MIP-1β, regulated on activation normal T-cell expressed and secreted, and interleukin (IL)-16. Treatment of activated HIV-1-infected CD8 + T cells with anti-CD3 monoclonal (M) antibody (Ab) and IL-15 induced strong production of interferon-γ (IFN-γ). Treatment of cells with anti-IL-12 MAb and IL-4 to induce a Tc1-to-Tc2 shift resulted in no change in viral production levels or IFN-γ production within the HIV-1-infected CD8 + T cell population. Initiation of a Tc2-to-Tc1 shift resulted in a 6-fold increase in HIV-1 replication and 2- to 3-fold higher levels of IFN-γ, demonstrating that infection can protect against a Tc1-to-Tc2 shift in CD8 + T cells. |
| Databáze: | OpenAIRE |
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