Clonal diversity of Haemophilus influenzae carriage isolated from under the age of 6 years children

Autor: Farzaneh Hosseini, Seyed Fazlollah Mousavi, Fahimeh Shooraj, Bahman Mirzaei
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Haemophilus Infections
medicine.drug_class
Tetracycline
030106 microbiology
030231 tropical medicine
Antibiotics
lcsh:Medicine
Microbial Sensitivity Tests
Biology
Iran
medicine.disease_cause
Antimicrobial resistance
General Biochemistry
Genetics and Molecular Biology
Microbiology
Haemophilus influenzae
Genomic analysis
03 medical and health sciences
0302 clinical medicine
Antibiotic resistance
Species Specificity
Levofloxacin
Ampicillin
Nasopharynx
medicine
Pulsed-field gel electrophoresis
Cluster Analysis
Humans
lcsh:Science (General)
lcsh:QH301-705.5
lcsh:R
Genetic Variation
Hemophilus influenzae
General Medicine
PFGE
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Research Note
Carriage
lcsh:Biology (General)
Child
Preschool
Carrier State
Female
medicine.drug
lcsh:Q1-390
Zdroj: BMC Research Notes, Vol 12, Iss 1, Pp 1-7 (2019)
BMC Research Notes
ISSN: 1756-0500
Popis: ObjectivesPharyngeal carriers such asH.influenzaeseem to constitute the only reservoir and probably the only transmission vehicle of the invasive disease. The aims of this study were to estimate the prevalence ofH. influenzaecarriage, to characterize antibiotic susceptibility, and to explore genetic diversity ofH. influenzaeisolates. Sampling was carried out as nasopharynx swabs among children less than 6 years old volunteers. After traditional biochemical tests, isolates were confirmed by targetingomp6sequence. Following the susceptibility tests, genomic diversity of strains was analyzed by Pulsed-Field Gel Electrophoresis procedure.ResultsOut of 328 nasopharynx swabs, 73 strains were identified as H.influenzae. AmongH. influenzaeisolates, resistance to chloramphenicol (42%) and ampicillin (43%) was observed. Levofloxacin is the most effective antibiotic and the least effect belonged to tetracycline. By genomic analysis of selectedH.influenza, 28 PFGE patterns were achieved among which 11 patterns included at least 2 strains. All strains clustered into 25 different clones. The dendrogram analysis of the isolatedH.influenzaestrains showed that some of these strains had a clonal relationship and common genetic origin. According to our results, antibiotic resistance didn’t show any significant correlation with the clonality of strains.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje