Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe

Autor: Sarah L. Long, Gerald F. Fitzgerald, Catherine Stanton, R. Paul Ross, Jerry M. Wells, Ellen H. Stolte, Cormac G. M. Gahan, Lis E. E. London, Susan A. Joyce, Paul M. Ryan, Noel M. Caplice
Přispěvatelé: Teagasc Walsh Fellowship Programme, European Union, Enterprise Ireland, Science Foundation Ireland, CF/2013/3030A/B, SFI/12/RC/2273, Ryan, Paul M [0000-0001-7251-6725], Joyce, Susan A [0000-0003-4771-3123], Gahan, Cormac GM [0000-0003-2385-8192], Ross, R Paul [0000-0003-4876-8839], Caplice, Noel M [0000-0001-5445-1016], Apollo - University of Cambridge Repository
Rok vydání: 2019
Předmět:
Limosilactobacillus reuteri
Swine
Lactobacillus mucosae
lcsh:QR1-502
lcsh:Microbiology
Mice
Lactobacillus
Gene expression
Bile salt hydrolase (BSH)
Bile
Hypercholesterolaemia
0303 health sciences
biology
Bile acid
Hydrolysis
Polysaccharides
Bacterial

food and beverages
CVD
3. Good health
Interleukin-10
Cardiovascular Diseases
Tumor necrosis factor alpha
Mannose Receptor
Research Article
Microbiology (medical)
medicine.drug_class
Receptors
Cell Surface

Nitric Oxide
Microbiology
Amidohydrolases
Immunomodulation
03 medical and health sciences
Immune system
Exopolysaccharide
medicine
Animals
Lectins
C-Type

Host-Microbe Interactomics
VLAG
030306 microbiology
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophages
Glycosyltransferases
biology.organism_classification
In vitro
Lactobacillus reuteri
Mannose-Binding Lectins
RAW 264.7 Cells
WIAS
Zdroj: BMC Microbiology
BMC Microbiology, Vol 19, Iss 1, Pp 1-13 (2019)
BMC Microbiology 19 (2019) 1
BMC Microbiology, 19(1)
ISSN: 1471-2180
Popis: Background Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model. This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease. Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity. The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587. Results Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro. Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids. In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages. Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription. Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses. Conclusions This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype. Electronic supplementary material The online version of this article (10.1186/s12866-019-1403-0) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE