Influences of Dryopteris crassirhizoma Extract on the Viability, Growth and Virulence Properties of Streptococcus mutans
| Autor: | Jae-Gyu Jeon, Jeong-Eun Kim, Santosh Pandit, Suk-Ho Ban |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Dryopteris
Dryopteris crassirhizoma virulence properties Pharmaceutical Science Virulence Microbial Sensitivity Tests Biology Plant Roots Article Bacterial Adhesion Permeability Analytical Chemistry Microbiology Streptococcus mutans Bacterial Proteins Drug Discovery Phytochemical composition Physical and Theoretical Chemistry Sugar Incubation Pathogen Microbial Viability Plant Extracts Methanol Organic Chemistry Hydrogen-Ion Concentration biology.organism_classification Anti-Bacterial Agents Proton-Translocating ATPases Glucosyltransferases Chemistry (miscellaneous) Solvents Molecular Medicine Glycolysis Bacteria |
| Zdroj: | Molecules; Volume 17; Issue 8; Pages: 9231-9244 Molecules |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules17089231 |
| Popis: | Dryopteris crassirhizoma is traditionally used as an herbal remedy for various diseases, and has been identified in a previous study as a potential anti-caries agent. In this study, the effect of a methanol extract of D. crassirhizoma on the viability, growth and virulence properties of Streptococcus mutans, a cariogenic dental pathogen, was investigated. In addition, the phytochemical composition of the extract was analyzed. The extract showed bactericidal and bacteriostatic activity against oral bacteria (MIC and MBC of S. mutans: 62.5 and 250 μg/mL, respectively). At two times the MBC, the extract significantly eliminated S. mutans up to 99.9% after 1 h incubation. The extract also dose-dependently reduced growth rates of S. mutans at sub-MIC levels. Furthermore, at sub-MIC levels, virulence properties (acid production, acid tolerance, glucosyltransferase activity and sucrose-dependent adherence) of S. mutans were also inhibited in a dose-dependent manner. GC-MS analysis revealed the presence of mono and disaccharides (44.9%), fatty acids (12.3%) and sugar alcohols (6.8%) in the extract. These data indicate that the extract might be useful for the control of dental caries. |
| Databáze: | OpenAIRE |
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