T-Cell Retargeting Using Bispecific Monoclonal Antibodies in a Rat Colon Carcinoma Model. I. Significant Bispecific Lysis of Syngeneic Colon Carcinoma CC531 Is Critically Dependent on Prolonged Preactivation of Effector T-Lymphocytes by Immobilized Anti-T-Cell Receptor Antibody
| Autor: | G. J. Fleuren, G. D. M. Beun, J. D. H. Van Eendenburg, W.E. Corver, C.J.H. van de Velde |
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| Rok vydání: | 1992 |
| Předmět: |
Cytotoxicity
Immunologic Male Cancer Research medicine.drug_class CD8 Antigens T-Lymphocytes T cell Immunology Receptors Antigen T-Cell chemical and pharmacologic phenomena In Vitro Techniques Biology Lymphocyte Activation Monoclonal antibody Antibody Specificity medicine Animals Immunology and Allergy Cytotoxicity Antilymphocyte Serum Pharmacology Antibody-dependent cell-mediated cytotoxicity T-cell receptor Antibodies Monoclonal Receptors Interleukin-2 hemic and immune systems T lymphocyte Molecular biology Rats medicine.anatomical_structure Colonic Neoplasms biology.protein Female Antibody CD8 |
| Zdroj: | Journal of Immunotherapy. 11:238-248 |
| ISSN: | 1524-9557 |
| DOI: | 10.1097/00002371-199205000-00002 |
| Popis: | In order to develop a rat model that reflects human weakly or nonimmunogenic tumor-host relationships and allows investigation of T-cell retargeting with bispecific monoclonal antibodies in vivo, we prepared several mixed hybridomas. One fusion partner was the anti-rat-T-cell receptor (TCR)-framework hybridoma R73 and the others were hybridomas producing antibodies against CC531, a Wag rat colon carcinoma. Stimulation of Wag rat spleen cells with immobilized R73 mAb and rIL-2 yielded predominantly CD8 positive effector T-lymphocytes, which lysed control P815 target cells efficiently in R73-mediated reverse antibody-dependent cellular cytotoxicity (ADCC). The capacity of these effectors to cause significant hybrid antibody-mediated lysis of CC531 emerged several days later, was critically dependent on prolonged stimulation with immobilized R73, and was associated with increased N-alfa-benzyloxycarbonyl-L-lysine thiobenzyl esterase content. |
| Databáze: | OpenAIRE |
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