Effect of high-protein feeding on insulin secretion in mice injected with splenocytes from diabetic mice
| Autor: | J. C. Basabe, Y. B. Lombardo, L. M. Karabatas |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty High protein Insulin medicine.medical_treatment Low dose Diabetic mouse Biology Streptozotocin General Biochemistry Genetics and Molecular Biology Streptozocin Diabetes Mellitus Experimental Mice Inbred C57BL Mice Endocrinology Internal medicine Insulin Secretion Splenocyte medicine Animals Secretion Dietary Proteins Insulin secretion medicine.drug |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 211(1) |
| ISSN: | 0037-9727 |
| Popis: | We report here our study of the in vifro glucose-induced insulin secretion from mice fed with a high-protein diet and injected with mononuclear splenocytes from streptozotocin-diabetic syngeneic donors. Results show that transfer of "dia- betic" splenocytes caused significant diminutions in first phase of stimulated-insulin secretion. Nevertheless, insulin secretory levels attained in recipient mice fed with the high-protein diet were significantly higher than in mice fed with control diets. We also evaluated the effect of high-protein feeding on the capacity of "diabetic" splenocytes to transfer an impaired insulin secretion in recipient mice. For this pur- pose, splenocytes donor mice were fed with high-protein or control diets and injected with multiple low doses of streptozotocin. "Diabetic" splenocytes from high-protein, or control diets fed donors caused in recipient mice similar impairments in glucose- stimulated insulin secretion. Taken together, these results seem to support the hypothesis of a protective effect of a high-protein diet on the p-cell function. (P.S.E.B.M. 1996, Vol 21 11 |
| Databáze: | OpenAIRE |
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