Beneficial effects of hydrogen-rich saline against spinal cord ischemia-reperfusion injury in rabbits
| Autor: | Yi Zeng, Weining Xue, Yi Huang, Keliang Xie, Xiaowu Wang, Gu Gong, Hongguang Chen, Leshun Zhou |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Potassium Channels Time Factors Pharmacology Sodium Chloride medicine.disease_cause Dinoprost Proinflammatory cytokine Superoxide dismutase chemistry.chemical_compound Malondialdehyde Medicine Animals Molecular Biology Spinal cord injury Motor Neurons Neurologic Examination biology Dose-Response Relationship Drug business.industry Caspase 3 Spinal Cord Ischemia Superoxide Dismutase General Neuroscience medicine.disease Spinal cord Catalase Dose–response relationship Disease Models Animal Oxidative Stress medicine.anatomical_structure Neuroprotective Agents chemistry Anesthesia Reperfusion Injury biology.protein Cytokines Neurology (clinical) Acyl Coenzyme A Rabbits business Reperfusion injury Oxidative stress Developmental Biology Hydrogen |
| Zdroj: | Brain research. 1517 |
| ISSN: | 1872-6240 |
| Popis: | Hydrogen-rich saline (HS) is reported to be a new therapeutic agent in ischemia-reperfusion (I/R)-induced organ damage. The present study was designed to investigate the beneficial effects of HS against spinal cord I/R injury and its associated mechanisms. Spinal cord ischemia was induced by infrarenal aortic occlusion for 20min in male New Zealand white rabbits. Different doses of HS were intravenously (i.v.) administered at 5min before or after the beginning of reperfusion. Moreover, the roles of mitochondrial ATP-sensitive potassium channels (mitoKATP), oxidative stress, inflammatory cytokines and apoptosis was assessed. Here, we found that I/R-challenged rabbits exhibited significant spinal cord injury characterized by the decreased numbers of normal motor neurons and hind-limb motor dysfunction, which was significantly ameliorated by 5mL/kg and 10mL/kg HS treatment before reperfusion or 10mL/kg HS treatment after reperfusion. However, the protective effects of HS treatment in spinal cord I/R injury were partially abolished by the selective mitoKATP channel blocker 5-hydroxydecanoate (5-HD). Moreover, we showed that the beneficial effects of 10mL/kg HS treatment against spinal cord I/R damage were associated with the decreased levels of oxidative products [8-iso-prostaglandin F2α (8-iso-PGF2α) and malondialdehyde (MDA)] and pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-α) and high-mobility group box 1 (HMGB1)], as well as the increased activities of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)] in serum at 6h, 12h, 24h, 48h and 72h after reperfusion and in spinal cord at 72h after reperfusion. Furthermore, HS treatment (10mL/kg) reduced caspase-3 activity in the spinal cord of this model. Thus, HS may be an effective therapeutic agent for spinal cord I/R injury via activation of mitoKATP channels as well as reduction of oxidative stress, inflammatory cytokines and apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect