Detection of placental growth hormone variant and chorionic somatomammotropin ribonucleic acid expression in human trophoblastic neoplasms by reverse transcriptase-polymerase chain reaction
| Autor: | Peter A. Cattini, Aristides Lytras, Janice G. Dodd, Margaret E. Bock |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Placenta Molecular Sequence Data Gene Expression Somatomammotropin Biology Polymerase Chain Reaction behavioral disciplines and activities chemistry.chemical_compound Endocrinology Human placental lactogen Pregnancy Internal medicine mental disorders Gene expression Tumor Cells Cultured medicine Humans Choriocarcinoma Gene Base Sequence Genetic Variation RNA RNA-Directed DNA Polymerase Hydatidiform Mole Placental Lactogen medicine.disease Molecular biology Reverse transcriptase chemistry Growth Hormone Uterine Neoplasms embryonic structures Female sense organs hormones hormone substitutes and hormone antagonists DNA |
| Zdroj: | Endocrinology. 134:2461-2467 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Attempts to assess human placental GH variant (hGH-V) and chorionic somatomammotropin (hCS) RNA in choriocarcinoma cell lines have been hampered by low levels of expression and limited sensitivity of RNA blotting analysis. We examined human choriocarcinoma BeWo, JAR, and JEG-3 cell lines as well as samples of complete hydatidiform moles for expression of members of the human GH (hGH) gene family using reverse transcriptase-polymerase chain reaction. A single and common set of primers was designed and used to detect products of the hGH/hCS genes as well as distinguish processed RNA from any contaminating DNA. Transcripts from the hCS genes hCS-A and -B were distinguished from placental hGH variant (hGH-V) and hCS-like (hCS-L) gene RNA by diagnostic restriction digestion of the polymerase chain reaction products. The expected pattern of hGH/hCS RNA expression was detected in term placenta, where hCS and hGH-V/hCS-L transcripts represented approximately 95% and approximately 5% of the total hGH/hCS RNA, respectively. The level of hCS RNA varied from 22-99% of the total hGH/hCS RNA in the neoplastic trophoblast samples, and variable levels of hGH-V and hCS-L RNA were also observed. In choriocarcinoma JAR cells, hGH-V RNA represented approximately 78% of the total hGH/hCS RNA compared to approximately 22% for hCS. Further, although low hCS-L RNA levels (1%) were found in term placenta and two of the hydatidiform moles, hCS-L transcripts represented 11% of the total hGH/hCS RNA in a third hydatidiform mole. Finally, in contrast to the detection of variable levels of hCS-L RNA in term placenta and hydatidiform mole samples, no hCS-L transcripts were detected in the three choriocarcinoma cell lines examined. These patterns reflect either deregulated hGH/hCS gene expression in neoplastic trophoblasts or differences that accompany the process of differentiation of trophoblast subpopulations. Regardless, this suggests that the control of hGH-V and hCS-L gene expression is distinct from that of the hCS-A and hCS-B genes and raises questions about the possible involvement of hGH/hCS family members in the pathology of placental abnormalities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|