Analysis of miRNA signatures in CSF identifies upregulation of miR-21 and miR-146a/b in patients with multiple sclerosis and active lesions

Autor: Lluís Ramió-Torrentà, Luisa M. Villar, Jordi Tomas-Roig, René Robles-Cedeño, Gemma Reverter, José Manuel Fernández-Real, Maria Buxó, Naiara Celarain, Francisco Ortega, María Muñoz-San Martín, Ester Quintana, Imma Gomez, H Perkal
Rok vydání: 2019
Předmět:
Zdroj: Journal of Neuroinflammation, Vol 16, Iss 1, Pp 1-10 (2019)
Journal of Neuroinflammation, 2019, vol. 16, art. núm. 220
Articles publicats (D-CM)
DUGiDocs – Universitat de Girona
instname
Journal of Neuroinflammation
ISSN: 1742-2094
DOI: 10.1186/s12974-019-1590-5
Popis: Background MicroRNAs (miRNAs) have been reported as deregulated in active brain lesions derived from patients with multiple sclerosis (MS). In there, these post-transcriptional regulators may elicit very important effects but proper identification of miRNA candidates as potential biomarkers and/or therapeutic targets is scarcely available. Objective The aim of the study was to detect the presence of a set of candidate miRNAs in cell-free cerebrospinal fluid (CSF) and to determine their association with gadolinium-enhancing (Gd+) lesions in order to assess their value as biomarkers of MS activity. Methods Assessment of 28 miRNA candidates in cell-free CSF collected from 46 patients with MS (26 Gd+ and 20 Gd− patients) was performed by TaqMan assays and qPCR. Variations in their relative abundance were analyzed by the Mann-Whitney U test and further evaluated by receiver operating characteristic (ROC) analysis. Signaling pathways and biological functions of miRNAs were analyzed using bioinformatic tools (miRTarBase, Enrichr, REVIGO, and Cytoscape softwares). Results Seven out of 28 miRNA candidates were detected in at least 75% of CSF samples. Consistent increase of miR-21 and miR-146a/b was found in Gd+ MS patients. This increase was in parallel to the number of Gd+ lesions and neurofilament light chain (NF-L) levels. Gene Ontology enrichment analysis revealed that the target genes of these miRNAs are involved in biological processes of key relevance such as apoptosis, cell migration and proliferation, and in cytokine-mediated signaling pathways. Conclusion Levels of miR-21 and miR-146a/b in cell-free CSF may represent valuable biomarkers to identify patients with active MS lesions.
Databáze: OpenAIRE
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