Osmotic stress, glucose transport capacity and consequences for glutamate overproduction in Corynebacterium glutamicum
| Autor: | Marson Raherimandimby, Hélène Dominguez, Muriel Cocaign-Bousquet, Pierre Gourdon, Nic D. Lindley |
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| Přispěvatelé: | SOREDAB sa, Université d'Antananarivo, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Unité mixte de recherche biotechnologies bioprocédés, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Osmotic shock
Glucose uptake Cell Culture Techniques Glutamic Acid Mannose Bioengineering Corynebacterium Biology Applied Microbiology and Biotechnology Corynebacterium glutamicum 03 medical and health sciences chemistry.chemical_compound Osmotic Pressure 030304 developmental biology 0303 health sciences Osmotic concentration 030306 microbiology Phosphotransferases Glucose transporter General Medicine Water-Electrolyte Balance Glucose [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Biochemistry Fermentation Energy source Biotechnology |
| Zdroj: | Journal of Biotechnology Journal of Biotechnology, Elsevier, 2003, 104 (1-3), pp.77-85. ⟨10.1016/S0168-1656(03)00165-2⟩ Journal of Biotechnology, 2003, 104 (1-3), pp.77-85. ⟨10.1016/S0168-1656(03)00165-2⟩ |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/S0168-1656(03)00165-2⟩ |
| Popis: | International audience; Glucose uptake by Corynebacterium glutamicum is predominantly assured by a mannose phosphotransferase system (PTS) with a high affinity for glucose (Km = 0.35 mM). Mutants selected for their resistance to 2-deoxyglucose (2DG) and lacking detectable PEP-dependent glucose-transporting activity, retained the capacity to grow on media in which glucose was the only carbon and energy source, albeit at significantly diminished rates, due to the presence of a low affinity (Ks = 11 mM) non-PTS uptake system. During growth in media of different osmolarity, specific rates of glucose consumption and of growth of wild type cells were diminished. Cell samples from these cultures were shown to possess similar PTS activities when measured under standard conditions. However, when cells were resuspended in buffer solutions of different osmolarity measurable PTS activity was shown to be dependent upon osmolarity. This inhibition effect was sufficient to account for the decreased rates of both sugar uptake and growth observed in fermentation media of high osmolarity. The secondary glucose transporter was, however, not influenced by medium osmolarity. During industrial fermentation conditions with accumulation of glutamic acid and the corresponding increase in medium osmolarity, similar inhibition of the sugar transport capacity was observed. This phenomenon provokes a major process constraint since the decrease in specific rates leads to an increasing proportion of sugar catabolised for maintenance requirements with an associated decrease in product yields. (C) 2003 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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