SNAREs in opposing bilayers interact in a circular array to form conducting pores
| Autor: | Sang-Joon Cho, Katherine T. Rognlien, Bhanu P. Jena, Jin Ah Cho, J. K. Heinrich Hörber, Marie L. Kelly |
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| Rok vydání: | 2002 |
| Předmět: |
Time Factors
Lipid Bilayers Biophysics Vesicular Transport Proteins Microscopy Atomic Force Biophysical Phenomena Cell membrane 03 medical and health sciences 0302 clinical medicine medicine Lipid bilayer 030304 developmental biology 0303 health sciences Chemistry Vesicle Cell Membrane Lipid bilayer fusion Membrane Proteins Membranes Artificial Interbilayer forces in membrane fusion Recombinant Proteins Cell biology Electrophysiology medicine.anatomical_structure Membrane protein Porosome Aluminum Silicates biological phenomena cell phenomena and immunity SNARE Proteins 030217 neurology & neurosurgery Protein Binding Research Article |
| Zdroj: | Biophysical journal. 83(5) |
| ISSN: | 0006-3495 |
| Popis: | The process of fusion at the nerve terminal is mediated via a specialized set of proteins in the synaptic vesicles and the presynaptic membrane. Three soluble N-ethylmaleimide-sensitive factor (NSF)-attachment protein receptors (SNAREs) have been implicated in membrane fusion. The structure and arrangement of these SNAREs associated with lipid bilayers were examined using atomic force microscopy. A bilayer electrophysiological setup allowed for measurements of membrane conductance and capacitance. Here we demonstrate that the interaction of these proteins to form a fusion pore is dependent on the presence of t-SNAREs and v-SNARE in opposing bilayers. Addition of purified recombinant v-SNARE to a t-SNARE-reconstituted lipid membrane increased only the size of the globular t-SNARE oligomer without influencing the electrical properties of the membrane. However when t-SNARE vesicles were added to a v-SNARE membrane, SNAREs assembles in a ring pattern and a stepwise increase in capacitance, and increase in conductance were observed. Thus, t- and v-SNAREs are required to reside in opposing bilayers to allow appropriate t-/v-SNARE interactions leading to membrane fusion. |
| Databáze: | OpenAIRE |
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