Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing
| Autor: | Marion Fischer-Le Saux, Charles Manceau, Sophie Bonneau, Marie-Agnès Jacques, Salwa Essakhi |
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| Přispěvatelé: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Part of a program funded by the Region Pays de la Loire (Xanthost) and by the Plant Health and Environment Department of INRA (SPE 2008-0077-03), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Xanthomonas [SDV]Life Sciences [q-bio] Molecular Sequence Data Population genetics [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy 01 natural sciences Applied Microbiology and Biotechnology Host Specificity 03 medical and health sciences Monophyly Plant Microbiology Botany Plant Diseases 030304 developmental biology Genetics 0303 health sciences Ecology biology Phylogenetic tree Xanthomonas arboricola Plants biology.organism_classification Xanthomonas campestris Pathovar Genetic structure Multilocus sequence typing Multilocus Sequence Typing 010606 plant biology & botany Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (14), pp.4651-4668. ⟨10.1128/AEM.00050-15⟩ Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (14), pp.4651-4668. ⟨10.1128/aem.00050-15⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.00050-15⟩ |
| Popis: | Deep and comprehensive knowledge of the genetic structure of pathogenic species is the cornerstone on which the design of precise molecular diagnostic tools is built. Xanthomonas arboricola is divided into pathovars, some of which are classified as quarantine organisms in many countries and are responsible for diseases on nut and stone fruit trees that have emerged worldwide. Recent taxonomic studies of the genus Xanthomonas showed that strains isolated from other hosts should be classified in X. arboricola , extending the host range of the species. To investigate the genetic structure of X. arboricola and the genetic relationships between highly pathogenic strains and strains apparently not relevant to plant health, we conducted multilocus sequence analyses on a collection of strains representative of the known diversity of the species. Most of the pathovars were clustered in separate monophyletic groups. The pathovars pruni, corylina, and juglandis, responsible for pandemics in specific hosts, were highly phylogenetically related and clustered in three distinct clonal complexes. In contrast, strains with no or uncertain pathogenicity were represented by numerous unrelated singletons scattered in the phylogenic tree. Depending on the pathovar, intra- and interspecies recombination played contrasting roles in generating nucleotide polymorphism. This work provides a population genetics framework for molecular epidemiological surveys of emerging plant pathogens within X. arboricola . Based on our results, we propose to reclassify three former pathovars of Xanthomonas campestris as X. arboricola pv. arracaciae comb. nov., X. arboricola pv. guizotiae comb. nov., and X. arboricola pv. zantedeschiae comb. nov. An emended description of X. arboricola Vauterin et al. 1995 is provided. |
| Databáze: | OpenAIRE |
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