Basic Fibroblast Growth Factor Is Upregulated in Hibernating Myocardium
| Autor: | Peter K. Smith, Kevin P. Landolfo, Monica L. Smith, John E. Scarborough, Brian H. Annex, Luis H. Diodato, Anne M. Pippen, Patrick W. Domkowski |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Swine Angiogenesis medicine.medical_treatment Basic fibroblast growth factor Ischemia Endogeny chemistry.chemical_compound Reference Values Internal medicine Animals Medicine cardiovascular diseases Myocardial Stunning Hibernating myocardium business.industry Myocardium Growth factor medicine.disease Pathophysiology Up-Regulation Cytokine Endocrinology chemistry cardiovascular system Swine Miniature Fibroblast Growth Factor 2 Surgery medicine.symptom business |
| Zdroj: | Journal of Surgical Research. 107:119-123 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.2002.6475 |
| Popis: | Ischemia is known to be a potent stimulus for the upregulation of angiogenic growth factors, such as basic fibroblast growth factor (bFGF). While previous investigations have shown that many angiogenic growth factors are upregulated in animal models of myocardial ischemia, the models used are limited in their ability to produce stable ischemia beyond a few weeks. Our laboratory uses a stable model of hibernating myocardium where later time points may be examined. Therefore, the goal of this study was to examine bFGF protein levels in the myocardium at baseline and 3 or 6 months following the onset of myocardial ischemia.A total of 18 miniswine were studied. Basal endogenous levels of bFGF were measured in control animals (n = 6) immediately following sacrifice, while 12 other pigs underwent a 90% left circumflex artery occlusion with documented hibernating myocardium by positron emission tomography ((13)N-ammonia) and dobutamine stress echocardiography. These animals were studied at 3 (n = 7) and 6 months (n = 5) postoperatively. At sacrifice, six 3 x 3 mm samples were harvested from the left circumflex (hibernating) myocardium. Basic FGF levels (picograms per microgram of protein) were determined using ELISA kits.Basic FGF protein levels 3 months after the creation of hibernating myocardium were three times greater than in nonischemic control animals (P0.05), while levels at 6 months were increased sixfold compared to control animals (P0.05 versus both control and 3-month groups).Endogenous bFGF production is upregulated at 3 and 6 months in hibernating porcine myocardium. The angiogenic effects of exogenous bFGF delivered into ischemic myocardium with varying levels of endogenous growth factors must be determined. |
| Databáze: | OpenAIRE |
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