Direct growth of few-layer graphene on AlN-based resonators for high-sensitivity gravimetric biosensors
| Autor: | Marta Clement, Bruno Marco, Teona Mirea, Enrique Iborra, Lorena Gordillo-Dagallier, Jimena Olivares, José Miguel Escolano |
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| Rok vydání: | 2019 |
| Předmět: |
Streptavidin
Materials science General Physics and Astronomy 02 engineering and technology Chemical vapor deposition lcsh:Chemical technology lcsh:Technology 01 natural sciences Full Research Paper law.invention chemistry.chemical_compound symbols.namesake law Molecule Nanotechnology lcsh:TP1-1185 General Materials Science Electrical and Electronic Engineering lcsh:Science surface functionalization Telecomunicaciones lcsh:T 010405 organic chemistry business.industry Graphene graphene integration 021001 nanoscience & nanotechnology gravimetric biosensor lcsh:QC1-999 0104 chemical sciences Nanoscience chemistry Covalent bond symbols biomolecule detection Optoelectronics Surface modification lcsh:Q Electrónica 0210 nano-technology business Raman spectroscopy Biosensor lcsh:Physics |
| Zdroj: | Beilstein Journal of Nanotechnology, ISSN 2190-4286, 2019-04, Vol. 10 Archivo Digital UPM Universidad Politécnica de Madrid Beilstein Journal of Nanotechnology Beilstein Journal of Nanotechnology, Vol 10, Iss 1, Pp 975-984 (2019) |
| Popis: | We present the successful growth of few-layer graphene on top of AlN-based solidly mounted resonators (SMR) using a low-temperature chemical vapour deposition (CVD) process assisted by Ni catalysts, and its effective bio-functionalization with antibodies. The SMRs are manufactured on top of fully insulating AlN/SiO2 acoustic mirrors able to withstand the temperatures reached during the CVD growth of graphene (up to 650 °C). The active AlN films, purposely grown with the c-axis tilted, effectively excite shear modes displaying excellent in-liquid performance, with electromechanical coupling and quality factors of around 3% and 150, respectively, which barely vary after graphene integration. Raman spectra reveal that the as-grown graphene is composed of less than five weakly coupled layers with a low density of defects. Two functionalization protocols of the graphene are proposed. The first one, based on a covalent binding approach, starts with a low-damage O2 plasma treatment that introduces a controlled density of defects in graphene, including carboxylic groups. After that, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) chemistry is used to covalently bind streptavidin molecules to the surface of the sensors. The second functionalization protocol is based on the non-covalent bonding of streptavidin on hydrophobic graphene surfaces. The two protocols end with the effective bonding of biotinylated anti-IgG antibodies to the streptavidin, which leaves the surface of the devices ready for possible IgG detection. |
| Databáze: | OpenAIRE |
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