Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies

Autor: Jaroslaw Grobelny, Malgorzata Krzyzowska, Emilia Tomaszewska, Anna Basa, Piotr Bąska, Piotr Orlowski, Grzegorz Celichowski, Katarzyna Winnicka, Emilia Szymańska
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Silver
Swine
Metal Nanoparticles
02 engineering and technology
Antiviral Agents
Catalysis
Silver nanoparticle
Article
Hydrogel
Polyethylene Glycol Dimethacrylate
Cell Line
Inorganic Chemistry
tannic acid modified silver nanoparticles
HSV 1/2
antiherpes activity
hydrogel
mucoadhesiveness
Carbopol 974P
03 medical and health sciences
chemistry.chemical_compound
Mice
In vivo
Adhesives
Tannic acid
Chlorocebus aethiops
Animals
Humans
Simplexvirus
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Virus quantification
Chemistry
Organic Chemistry
Herpes Simplex
General Medicine
Penetration (firestop)
021001 nanoscience & nanotechnology
In vitro
Computer Science Applications
Mice
Inbred C57BL
030104 developmental biology
Self-healing hydrogels
Biophysics
Female
0210 nano-technology
Tannins
Ex vivo
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 2; Pages: 387
ISSN: 1422-0067
Popis: Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were investigated for their rheological and mechanical properties followed with ex vivo mucoadhesive behavior to the porcine vaginal mucosa. Particular effort was made to evaluate the activity of nanoparticle-based hydrogels toward herpes simplex virus (HSV) type 1 and 2 infection in vitro in immortal human keratinocyte cell line and in vivo using murine model of HSV-2 genital infection. The effect of infectivity was determined by real time quantitative polymerase chain reaction, plaque assay, inactivation, attachment, penetration and cell-to-cell assessments. All analyzed nanoparticle-based hydrogels exhibited pseudoplastic and thixotropic properties. Viscosity and mechanical measurements of hydrogels were found to correlate with the mucoadhesive properties. The results confirmed the ability of nanoparticle-based hydrogels to affect viral attachment, impede penetration and cell-to-cell transmission, although profound differences in the activity evoked by tested preparations toward HSV-1 and HSV-2 were noted. In addition, these findings demonstrated the in vivo potential of tannic acid modified silver nanoparticle-based hydrogels for vaginal treatment of HSV-2 genital infection.
Databáze: OpenAIRE
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