Conformational Variability in Ground-State CFTR Lipoprotein Particle Cryo-EM Ensembles
| Autor: | Luba A. Aleksandrov, Adrei A. Aleksandrov, Timothy J. Jensen, Joshua D. Strauss, Jonathan F. Fay |
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| Rok vydání: | 2022 |
| Předmět: |
Cystic Fibrosis
Lipoproteins Organic Chemistry Cryoelectron Microscopy Cystic Fibrosis Transmembrane Conductance Regulator General Medicine Catalysis Computer Science Applications Inorganic Chemistry Adenosine Triphosphate cryo-EM CFTR cystic fibrosis electrophysiology SMALP lipids Humans Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 16; Pages: 9248 |
| ISSN: | 1422-0067 |
| Popis: | Cystic fibrosis transmembrane regulator (CFTR) is a dynamic membrane protein belonging to the ABC transporter family. It is unusual within this family as it is an ion channel, as opposed to a transporter. Activation of CFTR requires ATP and phosphorylation by PKA, and dysregulation of CFTR mediated salt and water homeostasis can lead to cystic fibrosis. Recent advancements in structural biological methods have led to more than 10 published CFTR structures, and, so far, all of these structures of CFTR, determined by cryo-EM, have been limited to detergent-purified protein preparations. To visualize CFTR in an environment that more closely represents its native membranous environment, we utilized two different lipoprotein particle encapsulation techniques: one in which the ion channel is first purified and then reconstituted using the membrane scaffolding protein Saposin A and another that uses the solubilizing polymer Sokalan CP9 (DIBMA) to extract CFTR directly from membranes. Structures derived from these types of preparations may better correlate to their function, for instance, the single-channel measurements from membrane vesicles. |
| Databáze: | OpenAIRE |
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