T regulatory markers expression in unexplained recurrent spontaneous abortion
| Autor: | Bita Saifi, Mahmood Erfanian Ahmadpour, Mehdi Mehdizadeh, Rosita Vakili, Narges Valizade, Sanaz Ahmadi, Reza Aflatoonian, Fatemehsadat Amjadi, Nader Tajik, Seyed Abdolrahim Rezaee |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Abortion Habitual Luteal phase Luteal Phase Peripheral blood mononuclear cell T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Antigen Interquartile range Pregnancy Glucocorticoid-Induced TNFR-Related Protein TaqMan Medicine Cytotoxic T cell Humans CTLA-4 Antigen Embryo Implantation Cells Cultured 030219 obstetrics & reproductive medicine business.industry Obstetrics and Gynecology Interleukin-10 Interleukin 10 Cross-Sectional Studies CTLA-4 Pediatrics Perinatology and Child Health Immunology Leukocytes Mononuclear Female business Biomarkers 030215 immunology |
| Zdroj: | The journal of maternal-fetalneonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. 29(7) |
| ISSN: | 1476-4954 |
| Popis: | To evaluate expression of glucocorticoid-induced tumor necrosis factor receptor (GITR), cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and IL-10 in peripheral blood mononuclear cells (PBMCs) of 20 women with unexplained recurrent spontaneous abortion (URSA) compared to 20 normal non-pregnant women (NNP) during luteal phase in the window of implantation.Quantitative real-time PCR (qRT-PCR) was performed using the Taqman method for expression of GITR and SYBR Green method for expression of CTLA-4 and IL-10.Expression of CTLA-4 in the NNPs (median; interquartile range; 3; 1.8-10) was significantly higher than the URSAs (0.72; 0.26-3.81, p = 0.015). Expression of GITR in the NNPs (53; 10-139) was significantly higher than the URSAs (6; 3-27, p = 0.005). However, IL-10 expression in the URSAs was significantly higher than the NNPs, did not meet a significant value. A significant correlation was found between CTLA-4 and GITR expression in the study population (p = 0.0001).Expression of CTLA-4 and GITR were significantly down-regulated in the URSAs compared to NNPs at the window of implantation, which shows the essential role of Treg cells in creating an immunological privileged site for fetus as an allograft at the maternal-fetal interface by high expression levels of CTLA-4 and GITR during a normal pregnancy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|