Hepatic Kupffer cell blockade reduces mortality of acute hemorrhagic pancreatitis in mice
| Autor: | Howard A. Reber, John S. Lane, Beat Gloor, Karen E. Todd, M. P. N. Lewis |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Kupffer Cells Neutrophils medicine.medical_treatment Anti-Inflammatory Agents Enzyme-Linked Immunosorbent Assay Gadolinium Hemorrhage Gastroenterology Proinflammatory cytokine Mice Acute hemorrhagic pancreatitis Internal medicine medicine Animals Lung Saline Peroxidase Interleukin-6 Tumor Necrosis Factor-alpha business.industry Mortality rate Kupffer cell Interleukin-10 Blockade medicine.anatomical_structure Pancreatitis Acute Disease Immunology Female Surgery Tumor necrosis factor alpha Inflammation Mediators business Interleukin-1 |
| Zdroj: | Journal of Gastrointestinal Surgery. 2:430-435 |
| ISSN: | 1091-255X |
| DOI: | 10.1016/s1091-255x(98)80033-4 |
| Popis: | Inflammatory cytoklines derived from the liver may cause distant organ failure and death in severe pancreatitis. To minimize liver cytokine release, we studied the effects of Kupffer cell blockade on the mortality rate and severity of inflammation in a model of that disease. Thirty mice were divided into three groups. Group I received gadolinium chloride (l mg/100 g intravenously), which blocks Kupffer cell activity, and regular food. Groups 2 and 3 were fed a choline-deficient, ethionine-supplemented diet and developed severe pancreatitis. Group 2 (control) received intravenous saline solution, and group 3 received gadolinium chloride. Animals were killed at 72 hours. Serum levels of tumor necrosis factor-alpha and interleukin-1Beta, interleukin-6, and interleukin-10 were determined by enzyme-linked immunosorbent assay. Lung neutrophil infiltration was assessed by myeloperoxidase assay. Pancreatic inflammation was scored in a blinded manner. In a separate experiment, mortality rates were determined in saline- and gadolinium-treated animals (n=100). Gadolinium reduced the levels of all the cytoklines and lung myeloperoxidase (P0.05). Gadolinium also reduced the mortality rate (52% vs. 86%; P0.001). However, the degree of pancreatic inflammation was unchanged by gadolinium treatment. These data support the hypothesis that mortality in severe pancreatitis may in part be related to the secondary release of hepatic cytokines. |
| Databáze: | OpenAIRE |
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