Apocynin protects retina cells from ultraviolet radiation damage via inducing sirtuin 1
| Autor: | Jinsheng Hong, Chen Lin, Chuanshu Cai, Feng Liu, Weijian Zhang, Lihong Guo, Jianrong Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Retinal Ganglion Cells inorganic chemicals Programmed cell death Ultraviolet Rays Pharmaceutical Science Retinal Pigment Epithelium 02 engineering and technology medicine.disease_cause Retinal ganglion Cell Line Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sirtuin 1 medicine Animals Humans cardiovascular diseases Mice Inbred BALB C Retina Retinal pigment epithelium biology Acetophenones Retinal 021001 nanoscience & nanotechnology Cell biology Oxidative Stress medicine.anatomical_structure chemistry Gene Knockdown Techniques 030220 oncology & carcinogenesis Apocynin cardiovascular system biology.protein sense organs 0210 nano-technology Oxidative stress circulatory and respiratory physiology |
| Zdroj: | Journal of Drug Targeting. 28:330-338 |
| ISSN: | 1029-2330 1061-186X |
| DOI: | 10.1080/1061186x.2019.1663527 |
| Popis: | Direct exposure to Ultraviolet (UV) radiation causes progressive damages in retinal cells, which is one of the hypothetical mechanisms underlying age-related retinopathy or macular degeneration. The protective effects of Apocynin against UV damages were firstly tested in retinal pigment epithelium cells (RPEs) and retinal ganglion cells (RGCs). Subsequently the beneficial effect of Apocynin on mouse retinas against light damage were examined. Next, microarray profiling was used to identify the genes regulated by Apocynin in both RPEs and RGCs. A candidate gene was isolated for functional characterisation by knock-down study. Apocynin was shown to inhibit cell death, reduce oxidative stress and deoxyribonucleic acid damages in both RPEs and RGCs challenged with UV. Intravitreal application of Apocynin also improved retinal dysfunction caused by light damage. Sirtuin 1 (SIRT1) was identified as induced by Apocynin by microarray study. The induction was confirmed by realtime-PCR and western blotting. Knocking down SIRT1 antagonised the protective effect of Apocynin against UV damages in both RPEs and RGCs. Apocynin is a novel agent that shows both in vitro and in vivo efficacies against UV radiation induced retina damages. SIRT1 pathway is implicated in UV radiation protection of Apocynin in retinal cells. |
| Databáze: | OpenAIRE |
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