Protective Effects of Amarogentin against Carbon Tetrachloride-Induced Liver Fibrosis in Mice
| Autor: | Hang Zhao, Fang Wang, Ya Zhang, Tian Zhang, Si-Wang Wang, Hua Li, Wei Cao |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Liver Cirrhosis
0301 basic medicine mitogen-activated protein kinase Pharmaceutical Science Plant Roots Antioxidants Analytical Chemistry Mice chemistry.chemical_compound 0302 clinical medicine Fibrosis Malondialdehyde Drug Discovery Iridoids Tissue Distribution Gentiana Glycosides Carbon Tetrachloride liver fibrosis chemistry.chemical_classification amarogentin Glutathione peroxidase carbon tetrachloride α-smooth muscle actin Hydroxyproline Chemistry (miscellaneous) Molecular Medicine 030211 gastroenterology & hepatology Nucleotides Cyclic medicine.medical_specialty Down-Regulation Biology Article Cell Line Superoxide dismutase 03 medical and health sciences Albumins Internal medicine medicine Animals Humans Physical and Theoretical Chemistry Cyclic guanosine monophosphate Swertia Dose-Response Relationship Drug Plant Extracts Organic Chemistry Amarogentin medicine.disease Actins Mice Inbred C57BL Oxidative Stress 030104 developmental biology Endocrinology chemistry Carbon tetrachloride biology.protein Phytotherapy |
| Zdroj: | Molecules; Volume 22; Issue 5; Pages: 754 Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| Popis: | Amarogentin, a secoiridoid glycoside that is mainly extracted from Swertia and Gentiana roots, has been suggested to exhibit many biological effects, including anti-oxidative, anti-tumour, and anti-diabetic activities. The present study was designed to evaluate the protective effects of amarogentin on carbon tetrachloride-induced liver fibrosis in vivo and the underlying mechanism. Fibrosis was induced by subcutaneous injections of 6 mL/kg of 20% carbon tetrachloride (dissolved in olive oil) twice per week for seven weeks. Mice were orally treated with 25, 50, and 100 mg/kg amarogentin and with colchicine as a positive control. Biochemical assays and histopathological investigations showed that amarogentin delayed the formation of liver fibrosis; decreased alanine aminotransferase, aspartate aminotransferase, malondialdehyde and hydroxyproline levels; and increased albumin, cyclic guanosine monophosphate, glutathione peroxidase, and superoxide dismutase levels. Moreover, amarogentin exhibited downregulation of α-smooth muscle actin and transforming growth factor-β1 levels in immunohistochemical and Western blot analyses. The levels of phosphorylated extracellular regulated protein kinases, c-Jun N-terminal kinase, and p38 were also significantly reduced in all amarogentin-treated groups in a dose-dependent manner. These findings demonstrated that amarogentin exerted significant hepatoprotective effects against carbon tetrachloride-induced liver fibrosis in mice and suggested that the effect of amarogentin against liver fibrosis may be by anti-oxidative properties and suppressing the mitogen-activated protein kinase signalling pathway. |
| Databáze: | OpenAIRE |
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