A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity
Autor: | Anthony P. Neumann, Marcel Giesbers, Fabio Cumbo, Eva Miriam Buhl, Saravanan Devendran, Anja J. Taverne-Thiele, Thomas C. A. Hitch, Thomas Riedel, Neeraj Kumar, Sara B. Hernández, Tomas de Wouters, Tobias A. M. Gulder, Felipe Cava, Ilias Lagkouvardos, Sascha Rohn, Torey Looft, Till Strowig, Cathrin Spröer, Viktoria Heine, David Wylensek, Afrizal Afrizal, Klaus Neuhaus, Marzena Wyschkon, Krzysztof Flisikowski, Thomas Fischöder, Jerry M. Wells, Lindsey Ly, Till Robin Lesker, Tianzhe Liu, Markus Reichlin, Laura Navarro Suarez, Nicola Segata, Heidi L. Doden, Boyke Bunk, Thomas Clavel, Jason M. Ridlon, Garret Suen, Esther Wortmann, Valeria R. Parreira, Birte Abt, Jörg Overmann, Paul M. D'Agostino, Emma Allen-Vercoe, Angelika Schnieke, Lothar Elling, João M. P. Alves |
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Přispěvatelé: | HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany., Hitch, Thomas CA [0000-0003-2244-7412], Hernández, Sara B [0000-0002-8349-360X], M D'Agostino, Paul [0000-0002-8323-5416], Cumbo, Fabio [0000-0003-2920-5838], Looft, Torey [0000-0001-8066-6715], Suen, Garret [0000-0002-6170-711X], Allen-Vercoe, Emma [0000-0002-8716-327X], Bunk, Boyke [0000-0002-8420-8161], Neuhaus, Klaus [0000-0002-6020-2814], Segata, Nicola [0000-0002-1583-5794], Elling, Lothar [0000-0002-3654-0397], Strowig, Till [0000-0003-0185-1459], Overmann, Jörg [0000-0003-3909-7201], Clavel, Thomas [0000-0002-7229-5595], Apollo - University of Cambridge Repository |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Swine General Physics and Astronomy Gut flora Feces Clostridium Lactobacillus RNA Ribosomal 16S 80 and over Phylogeny Genetics Aged 80 and over Multidisciplinary biology Bacterial Genomics Biodiversity Intestines Multigene Family Female ddc:500 Wageningen Electron Microscopy Centre 16S Science 030106 microbiology Microbiology General Biochemistry Genetics and Molecular Biology Article Host Specificity Bacterial genetics Bile Acids and Salts 03 medical and health sciences Phylogenetics Life Science Animals Humans Host-Microbe Interactomics Bacteria Genes Bacterial Metagenome Gastrointestinal Microbiome Bacterial genomics Aged Ribosomal General Chemistry Ribosomal RNA biology.organism_classification Mikrobiologi 030104 developmental biology [Clostridium] scindens Genes Metagenomics RNA INTESTINO DE ANIMAL Microbiome |
Zdroj: | Nature Communications, 11(1) Nature communications England Nature Communications 11 (2020) 1 Nature Communications Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Nature Communications 11, 6389 (2020). doi:10.1038/s41467-020-19929-w Nature Communications, Vol 11, Iss 1, Pp 1-26 (2020) |
ISSN: | 2041-1723 |
Popis: | Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus, Fusobacterium, and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci, and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae. The strain collection, called ‘Pig intestinal bacterial collection’ (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota. The authors present a public collection of 117 bacterial isolates from the pig gut, including the description of 38 novel taxa. Interesting functions discovered in these organisms include a new fucosyltransferease and sactipeptide-like molecules encoded by biosynthetic gene clusters. |
Databáze: | OpenAIRE |
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