A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity

Autor: Anthony P. Neumann, Marcel Giesbers, Fabio Cumbo, Eva Miriam Buhl, Saravanan Devendran, Anja J. Taverne-Thiele, Thomas C. A. Hitch, Thomas Riedel, Neeraj Kumar, Sara B. Hernández, Tomas de Wouters, Tobias A. M. Gulder, Felipe Cava, Ilias Lagkouvardos, Sascha Rohn, Torey Looft, Till Strowig, Cathrin Spröer, Viktoria Heine, David Wylensek, Afrizal Afrizal, Klaus Neuhaus, Marzena Wyschkon, Krzysztof Flisikowski, Thomas Fischöder, Jerry M. Wells, Lindsey Ly, Till Robin Lesker, Tianzhe Liu, Markus Reichlin, Laura Navarro Suarez, Nicola Segata, Heidi L. Doden, Boyke Bunk, Thomas Clavel, Jason M. Ridlon, Garret Suen, Esther Wortmann, Valeria R. Parreira, Birte Abt, Jörg Overmann, Paul M. D'Agostino, Emma Allen-Vercoe, Angelika Schnieke, Lothar Elling, João M. P. Alves
Přispěvatelé: HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany., Hitch, Thomas CA [0000-0003-2244-7412], Hernández, Sara B [0000-0002-8349-360X], M D'Agostino, Paul [0000-0002-8323-5416], Cumbo, Fabio [0000-0003-2920-5838], Looft, Torey [0000-0001-8066-6715], Suen, Garret [0000-0002-6170-711X], Allen-Vercoe, Emma [0000-0002-8716-327X], Bunk, Boyke [0000-0002-8420-8161], Neuhaus, Klaus [0000-0002-6020-2814], Segata, Nicola [0000-0002-1583-5794], Elling, Lothar [0000-0002-3654-0397], Strowig, Till [0000-0003-0185-1459], Overmann, Jörg [0000-0003-3909-7201], Clavel, Thomas [0000-0002-7229-5595], Apollo - University of Cambridge Repository
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Swine
General Physics and Astronomy
Gut flora
Feces
Clostridium
Lactobacillus
RNA
Ribosomal
16S
80 and over
Phylogeny
Genetics
Aged
80 and over
Multidisciplinary
biology
Bacterial
Genomics
Biodiversity
Intestines
Multigene Family
Female
ddc:500
Wageningen Electron Microscopy Centre
16S
Science
030106 microbiology
Microbiology
General Biochemistry
Genetics and Molecular Biology
Article
Host Specificity
Bacterial genetics
Bile Acids and Salts
03 medical and health sciences
Phylogenetics
Life Science
Animals
Humans
Host-Microbe Interactomics
Bacteria
Genes
Bacterial
Metagenome
Gastrointestinal Microbiome
Bacterial genomics
Aged
Ribosomal
General Chemistry
Ribosomal RNA
biology.organism_classification
Mikrobiologi
030104 developmental biology
[Clostridium] scindens
Genes
Metagenomics
RNA
INTESTINO DE ANIMAL
Microbiome
Zdroj: Nature Communications, 11(1)
Nature communications
England
Nature Communications 11 (2020) 1
Nature Communications
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Nature Communications 11, 6389 (2020). doi:10.1038/s41467-020-19929-w
Nature Communications, Vol 11, Iss 1, Pp 1-26 (2020)
ISSN: 2041-1723
Popis: Our knowledge about the gut microbiota of pigs is still scarce, despite the importance of these animals for biomedical research and agriculture. Here, we present a collection of cultured bacteria from the pig gut, including 110 species across 40 families and nine phyla. We provide taxonomic descriptions for 22 novel species and 16 genera. Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes reveal prevalent and pig-specific species within Lactobacillus, Streptococcus, Clostridium, Desulfovibrio, Enterococcus, Fusobacterium, and several new genera described in this study. Potentially interesting functions discovered in these organisms include a fucosyltransferase encoded in the genome of the novel species Clostridium porci, and prevalent gene clusters for biosynthesis of sactipeptide-like peptides. Many strains deconjugate primary bile acids in in vitro assays, and a Clostridium scindens strain produces secondary bile acids via dehydroxylation. In addition, cells of the novel species Bullifex porci are coccoidal or spherical under the culture conditions tested, in contrast with the usual helical shape of other members of the family Spirochaetaceae. The strain collection, called ‘Pig intestinal bacterial collection’ (PiBAC), is publicly available at www.dsmz.de/pibac and opens new avenues for functional studies of the pig gut microbiota.
The authors present a public collection of 117 bacterial isolates from the pig gut, including the description of 38 novel taxa. Interesting functions discovered in these organisms include a new fucosyltransferease and sactipeptide-like molecules encoded by biosynthetic gene clusters.
Databáze: OpenAIRE
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