Reph, a Regulator of Eph Receptor Expression in the Drosophila melanogaster Optic Lobe
| Autor: | Brian Reed, Richard E. Dearborn, Tamar Karian, Yong Dai, Jessica Gray, Sam Kunes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Genetic Screens Gene Identification and Analysis lcsh:Medicine 0302 clinical medicine Drosophila Proteins Pattern Formation lcsh:Science Genetics 0303 health sciences Multidisciplinary Gene Expression Regulation Developmental Nuclear Proteins EPH receptor A2 3. Good health Cell biology Axon Guidance Drosophila melanogaster Female Signal transduction biological phenomena cell phenomena and immunity Research Article Signal Transduction DNA transcription Biology EPH receptor B2 Signaling Pathways Molecular Genetics 03 medical and health sciences EPH receptor A3 Developmental Neuroscience Ephrin Animals Gene Regulation Gene Networks 030304 developmental biology Receptors Eph Family lcsh:R Optic Lobe Nonmammalian Erythropoietin-producing hepatocellular (Eph) receptor Membrane Proteins Molecular Development biological factors Signaling Mutation lcsh:Q Ectopic expression Gene expression Gene Function Molecular Neuroscience Organism Development 030217 neurology & neurosurgery Genetic screen Developmental Biology Neuroscience |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 5, p e37303 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Receptors of the Eph family of tyrosine kinases and their Ephrin ligands are involved in developmental processes as diverse as angiogenesis, axon guidance and cell migration. However, our understanding of the Eph signaling pathway is incomplete, and could benefit from an analysis by genetic methods. To this end, we performed a genetic modifier screen for mutations that affect Eph signaling in Drosophila melanogaster. Several dozen loci were identified on the basis of their suppression or enhancement of an eye defect induced by the ectopic expression of Ephrin during development; many of these mutant loci were found to disrupt visual system development. One modifier locus, reph (regulator of eph expression), was characterized in molecular detail and found to encode a putative nuclear protein that interacts genetically with Eph signaling pathway mutations. Reph is an autonomous regulator of Eph receptor expression, required for the graded expression of Eph protein and the establishment of an optic lobe axonal topographic map. These results reveal a novel component of the regulatory pathway controlling expression of eph and identify reph as a novel factor in the developing visual system. |
| Databáze: | OpenAIRE |
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