Effects of Neurotrophic Factors on Growth and Glial Cell Alignment of Cultured Adult Spiral Ganglion Cells
| Autor: | Shaden Ali M Khalifa, Wei Liu, Ulla Friberg, Henrik Boström, Marja Boström, Helge Rask-Andersen |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Neurite Physiology animal diseases Guinea Pigs Cell Culture Techniques Neurotrophin-3 Speech and Hearing Neurotrophin 3 Neurotrophic factors Neurites medicine Glial cell line-derived neurotrophic factor Animals Humans Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Axon Spiral ganglion biology urogenital system Sensory Systems Cell biology medicine.anatomical_structure nervous system Otorhinolaryngology biology.protein Schwann Cells Spiral Ganglion Neuroscience GDNF family of ligands Neurotrophin |
| Zdroj: | Audiology and Neurotology. 15:175-186 |
| ISSN: | 1421-9700 1420-3030 |
| DOI: | 10.1159/000251915 |
| Popis: | Adult spiral ganglion cells were cultured in chorus to assess the influence of the neurotrophins brain-derived neurotrophic factor, neurotrophin 3 and glial cell line-derived neurotrophic factor (GDNF) on neurite growth and Schwann cell alignment. Over 1500 measurements were collected using each factor at 10 ng/ml and all three in combination. Evaluation was made with GDNF at concentrations of up to 100 ng/ml. Neurite dimensions were assessed at days 5, 7, 9 and 11 using a computer-based program (Axon Analyzer). GDNF had a strong effect on spiral ganglion cell growth almost attaining the level of all three factors in combination. GDNF increased glial cell alignment and nerve bundle formation. Results show the potential of GDNF to maintain and possibly restore auditory nerve integrity. |
| Databáze: | OpenAIRE |
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