Glucose metabolism by mouse cells (NCTC clone 929) under conditions of defined nutrition
| Autor: | C. Waymouth, Paul A. Kitos |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
chemistry.chemical_classification
Alanine Carbon Isotopes Chromatography Research Cell Biology Glutamic acid Metabolism Carbohydrate metabolism Biology Pentose phosphate pathway Culture Media Amino acid Lactic acid Tissue Culture Techniques Citric acid cycle Mice chemistry.chemical_compound Glucose chemistry Biochemistry Connective Tissue Animals Autoradiography Carbohydrate Metabolism |
| Zdroj: | Experimental Cell Research. 35:108-118 |
| ISSN: | 0014-4827 |
| Popis: | Clone 929 mouse cells were grown in a nutrient medium deficient in bicarbonate. The cells were exposed to specifically labeled 14C glucose and some of the radioactive products of metabolism were identified and measured. The cells metabolize initially one-fifth to one-quarter of the consumed glucose by the pentose pathway. They utilize the remainder of the sugar principally by the Embden-Meyerhof-Parnas pathway. Lactic acid and alanine are discharged into the medium in relatively large amounts. Further oxidation of some of the glycolytic products proceeds by the Krebs tricarboxylic acid cycle, from which glutamic acid is formed and accumulates. When the nutrient medium is deficient in the amino acids of close proximity to the Krebs cycle the 14C-glucose is metabolized in such a way that radioactive carbon dioxide and lactic acid accumulate more extensively and the amino acids less extensively than they do in the complete medium. |
| Databáze: | OpenAIRE |
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