Nerve fibre layer degeneration and retinal ganglion cell loss long term after optic nerve crush or transection in adult mice
| Autor: | Manuel Vidal-Sanz, Francisco M. Nadal-Nicolás, Francisco J. Valiente-Soriano, Manuel Salinas-Navarro, Marta Agudo-Barriuso, M.C. Sánchez-Migallón, Manuel Jiménez-López |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Retinal Ganglion Cells
Pathology medicine.medical_specialty genetic structures Cell Survival Nerve Crush medicine.medical_treatment Nerve fiber layer Optic disk Cell Count Biology Retinal ganglion Lesion Mice 03 medical and health sciences Cellular and Molecular Neuroscience Nerve Fibers 0302 clinical medicine Neurofilament Proteins medicine Animals Fluorescent Antibody Technique Indirect Transcription Factor Brn-3A Retina Axotomy eye diseases Sensory Systems Ophthalmology medicine.anatomical_structure Microscopy Fluorescence nervous system Retinal ganglion cell Optic Nerve Injuries Nerve Degeneration 030221 ophthalmology & optometry Optic nerve Female sense organs medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Experimental Eye Research. 170:40-50 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2018.02.010 |
| Popis: | We have investigated the long term effects of two different models of unilateral optic nerve (ON) lesion on retinal ganglion cells (RGCs) and their axons, in the injured and contralateral retinas of adult albino mice. Intact animals were used as controls. The left ON was intraorbitally crushed or transected at 0.5 mm from the optic disk and both retinas were analyzed at 2, 3, 5, 7, 14, 30, 45 or 90 days after injury. RGCs were immunoidentified with anti-Brn3a, and their axons with anti-highly phosphorylated axonal neurofilament subunit H (pNFH). After both lesions, RGC death in the injured retinas is first significant at day 3, and progresses quickly up to 7 days slowing down till 90 days. In the same retinas, the anatomical loss of RGC axons is not evident until day 30. However, by two days after both lesions there are changes in the expression pattern of pNFH: axonal beads, axonal club- or bulb-like formations, and pNFH+RGC somas. The number of pNFH+RGC somata peak at day 5 after either lesion and is significantly higher than in intact retinas at all time points. pNFH+RGC somata are distributed across the retina, in accordance with the pattern of RGC death which is diffuse and homogenous. In the contralateral retinas there is no RGC loss, but there are few pNFH+RGCs from day 2 to day 90. In conclusion, in albino mice, axotomy-induced RGC death precedes the loss of their intraretinal axons and occurs in two phases, a rapid and a slower, but steady, one. Injured retinas show similar changes in the pattern of pNFH expression and a comparable course of RGC loss. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect