Identification of candidate genes of nasopharyngeal carcinoma by bioinformatical analysis
| Autor: | Zhenfu Fu, Zhimin Ye, Fangzheng Wang, Fengqin Yan, Fujun Hu, Lei Wang, Tongxin Liu, Bin Li, Mingxiang Jiang |
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| Rok vydání: | 2019 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Candidate gene Microarray Proto-Oncogene Proteins c-jun Computational biology Biology Wnt-5a Protein Proto-Oncogene Proteins c-myb 03 medical and health sciences 0302 clinical medicine SOX2 Mesenchymal cell proliferation medicine Humans MYB Protein Interaction Maps General Dentistry Gene Nasopharyngeal Carcinoma Gene Expression Profiling Forkhead Box Protein M1 Computational Biology Nasopharyngeal Neoplasms 030206 dentistry Cell Biology General Medicine medicine.disease Gene Expression Regulation Neoplastic 030104 developmental biology Otorhinolaryngology Nasopharyngeal carcinoma FOXM1 |
| Zdroj: | Archives of Oral Biology. 106:104478 |
| ISSN: | 0003-9969 |
| Popis: | Objective This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. Methods Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC and normal samples. Functional and pathway enrichment analysis of the DEGs were performed. Protein-protein interaction network and gene-transcription factor regulatory network of DEGs were constructed. And the expression of hub genes in NPC was also validated based on the public database. Results A total of 16 up-regulated and 27 down-regulated genes were screened out from the microarray datasets. Functional and pathway enrichment analysis showed that DEGs were mostly enriched in positive regulation of angiogenesis, mesenchymal cell proliferation, cell surface and DNA binding, ECM-receptor interaction pathway, PI3K-Akt signaling pathway, and pathways in cancer. Five hub genes JUN, VEGFA, FOXM1, MYB, and WNT5A were identified from the protein-protein interaction network. Subsequently, the hub gene-transcription factor regulatory network revealed that STAT3, MYC, SOX2, RUNX2 present key relations with hub genes. The expression of these five hub genes were also validated to be differentially expressed among NPC and normal samples. Conclusions The current study indicated that the hub DEGs JUN, VEGFA, FOXM1, MYB, and WNT5A we identified might be potential therapeutic biomarkers of NPC. |
| Databáze: | OpenAIRE |
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