Gene expression in Escherichia coli biofilms
| Autor: | Thomas K. Wood, Dacheng Ren, Rick W. Ye, Laura A. Bedzyk, Stuart M. Thomas |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Biology
medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Gene expression Escherichia coli medicine Gene Heat-Shock Proteins Oligonucleotide Array Sequence Analysis Escherichia coli Proteins Gene Expression Profiling Biofilm Gene Expression Regulation Bacterial General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Molecular biology SOXS Genes Bacterial Biofilms Fimbriae Bacterial DNA microarray Bacteria Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 64:515-524 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-003-1517-y |
| Popis: | DNA microarrays were used to study the gene expression profile of Escherichia coli JM109 and K12 biofilms. Both glass wool in shake flasks and mild steel 1010 plates in continuous reactors were used to create the biofilms. For the biofilms grown on glass wool, 22 genes were induced significantly (por =0.05) compared to suspension cells, including several genes for the stress response ( hslS, hslT, hha, and soxS), type I fimbriae ( fimG), metabolism ( metK), and 11 genes of unknown function ( ybaJ, ychM, yefM, ygfA, b1060, b1112, b2377, b3022, b1373, b1601, and b0836). The DNA microarray results were corroborated with RNA dot blotting. For the biofilm grown on mild steel plates, the DNA microarray data showed that, at a specific growth rate of 0.05/h, the mature biofilm after 5 days in the continuous reactors did not exhibit differential gene expression compared to suspension cells although genes were induced at 0.03/h. The present study suggests that biofilm gene expression is strongly associated with environmental conditions and that stress genes are involved in E. coli JM109 biofilm formation. |
| Databáze: | OpenAIRE |
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