SENS-IS, a 3D reconstituted epidermis based model for quantifying chemical sensitization potency: Reproducibility and predictivity results from an inter-laboratory study
| Autor: | Imène-Sarah Henaoui, Claude Auriault, Pascal Barbry, Jean-Claude Ourlin, Elodie Boitel, Pierre Aeby, Hervé Groux, Jean-Luc Peiffer, Bernard Mari, Isabelle Fabre, Agnès Paquet, Françoise Cottrez, Ambre Vallauri |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Computer science Gene Expression Computational biology 010501 environmental sciences In Vitro Techniques Toxicology Animal Testing Alternatives Dermatitis Contact 01 natural sciences Models Biological 03 medical and health sciences medicine Potency Humans Statistical analysis Inter-laboratory Sensitization 0105 earth and related environmental sciences Reproducibility Skin sensitization Reproducibility of Results General Medicine Allergens 030104 developmental biology medicine.anatomical_structure Test set Test protocol Epidermis Laboratories |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 32 |
| ISSN: | 1879-3177 |
| Popis: | The SENS-IS test protocol for the in vitro detection of sensitizers is based on a reconstructed human skin model (Episkin) as the test system and on the analysis of the expression of a large panel of genes. Its excellent performance was initially demonstrated with a limited set of test chemicals. Further studies (described here) were organized to confirm these preliminary results and to obtain a detailed statistical analysis of the predictive capacity of the assay. A ring-study was thus organized and performed within three laboratories, using a test set of 19 blind coded chemicals. Data analysis indicated that the assay is robust, easily transferable and offers high predictivity and excellent within- and between-laboratories reproducibility. To further evaluate the predictivity of the test protocol according to Cooper statistics a comprehensive test set of 150 chemicals was then analyzed. Again, data analysis confirmed the excellent capacity of the SENS-IS assay for predicting both hazard and potency characteristics, confirming that this assay should be considered as a serious alternative to the available in vivo sensitization tests. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect