In Situ Formation of Au-Glycopolymer Nanoparticles for Surface-Enhanced Raman Scattering-Based Biosensing and Single-Cell Immunity
| Autor: | Zi-Chun Chia, Ting-Yu Cheng, Chih Chia Huang, Horng Long Cheng, Li-Xing Yang, Tzu-Chi Huang, Ya-Jyun Chen, Yi-Syun Fang, Fei-Ting Hsu, Yu Ying Chen, Ying Jan Wang |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | ACS Applied Materials & Interfaces. 13:52295-52307 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.1c13647 |
| Popis: | Successful synthesis of glyconanoparticles has attracted much attention due to their various biointeractive capabilities, but it is still a challenge to understand different single-cell responses to exogenous particles among cell populations. Herein, we designed polyaniline-containing galactosylated gold nanoparticles (Au@PGlyco NPs) via in situ polymerization of ortho-nitrophenyl-β-galactoside assisted by Au nucleation. The nanogold-carrying polyaniline block produced electromagnetic enhancement in surface-enhanced Raman scattering (SERS). The underlying polymerization mechanism of ortho-nitrophenyl compounds via the formation of Au nanoparticles was investigated. Depending on how the galactoside moiety reacted with β-galactosidase derived from bacteria, the Au@PGlyco NPs-mediated SERS biosensor could detect low amounts of bacteria (∼1 × 102 CFU/mL). In addition, a high accumulation of Au@PGlyco NPs mediated the immune response of tumor-associated M2 macrophages to the immunogenic M1 macrophage transition, which was elicited by reactive oxygen levels biostimulation using single-cell SERS-combined fluorescence imaging. Our study suggested that Au@PGlyco NPs may serve as a biosensing platform with the labeling capacity on galactose-binding receptors expressed cell and immune regulation. |
| Databáze: | OpenAIRE |
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