Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation
| Autor: | Nina J. Drize, Tamara Sorokina, Alexey Bigildeev, Valeri G. Savchenko, Nataliya Petinati, Larisa A. Kuzmina, Elena N. Parovichnikova, Irina N. Shipounova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Myeloid Stromal cell Time Factors Adolescent medicine.medical_treatment Gene Expression Hematopoietic stem cell transplantation Colony-Forming Units Assay 03 medical and health sciences Young Adult Bone Marrow hemic and lymphatic diseases medicine Tumor Microenvironment Humans Transplantation Homologous Postoperative Period Cells Cultured Chemotherapy Tumor microenvironment business.industry Mesenchymal stem cell Hematopoietic Stem Cell Transplantation Myeloid leukemia Mesenchymal Stem Cells Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma Leukemia Myeloid Acute 030104 developmental biology medicine.anatomical_structure Oncology Immunology Preoperative Period Female Bone marrow business Biomarkers |
| Zdroj: | Leukemialymphoma. 58(2) |
| ISSN: | 1029-2403 |
| Popis: | Bone marrow (BM) derived adult multipotent mesenchymal stromal cells (MMSCs) and fibroblast colony-forming units (CFU-Fs) of 20 patients with acute myeloid leukemia (AML) and 15 patients with acute lymphoblastic leukemia (ALL) before and during 1 year after receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) were studied. The growth characteristics of MMSCs of all patients before allo-HSCT were not altered; however, relative expression level (REL) of some genes in MMSCs, but not in CFU-Fs, from AML and ALL patients significantly changed. After allo-HSCT, CFU-F concentration and MMSC production were significantly decreased for 1 year; REL of several genes in MMSCs and CFU-F-derived colonies were also significantly downregulated. Thus, chemotherapy that was used for induction of remission did not impair the function of stromal precursors, but gene expression levels were altered. Allo-HSCT conditioning regimens significantly damaged MMSCs and CFU-Fs, and the effect lasted for at least 1 year. |
| Databáze: | OpenAIRE |
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